Reduced expression of uroplakin 1A is associated with the poor prognosis of gastric adenocarcinoma patients

The aim of this study was to investigate the expression and prognostic significance of Uroplakin1A (UPK1A) in gastric adenocarcinoma patients. Functional studies were also analyzed in vitro. Real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical (IHC) staining methods were u...

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Veröffentlicht in:	PloS one 2014-04, Vol.9 (4), p.e93073-e93073
Hauptverfasser:	Zheng, Yan, Wang, Dan-dan, Wang, Wei, Pan, Ke, Huang, Chun-yu, Li, Yuan-fang, Wang, Qi-jing, Yuan, Shu-qiang, Jiang, Shan-shan, Qiu, Hai-bo, Chen, Yong-ming, Zhang, Xiao-fei, Zhao, Bai-wei, Mai, Cong, Xia, Jian-chuan, Zhou, Zhi-wei
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma Adenocarcinoma - metabolism Adenocarcinoma - mortality Adenocarcinoma - secondary Apoptosis Biology and Life Sciences Biomarkers Blotting, Western Cancer Cancer therapies Cell Adhesion Cell Cycle Cell migration Cell Movement Cell Proliferation Correlation analysis Esophagus Female G1 phase Gastric cancer Gene expression Hazards Humans Immunoenzyme Techniques In vitro methods and tests Kinases Laboratories Lymphatic Metastasis Male Medical prognosis Medicine and Health Sciences Metastases Oncology Paraffin Patients Phosphorylation Prognosis Quality Real-Time Polymerase Chain Reaction Research and Analysis Methods Reverse Transcriptase Polymerase Chain Reaction Risk factors RNA, Messenger - genetics Staining Stomach - metabolism Stomach cancer Stomach Neoplasms - metabolism Stomach Neoplasms - mortality Stomach Neoplasms - pathology Surgery Survival Tumor Cells, Cultured Uroplakin Ia - genetics Uroplakin Ia - metabolism Western blotting
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Zusammenfassung:	The aim of this study was to investigate the expression and prognostic significance of Uroplakin1A (UPK1A) in gastric adenocarcinoma patients. Functional studies were also analyzed in vitro. Real-time quantitative PCR (RT-qPCR), western blotting, and immunohistochemical (IHC) staining methods were used to analyze the expression of UPK1A in primary gastric adenocarcinoma tissue samples. Compared with matched adjacent non-tumor, the expression of UPK1A in fresh surgical specimens was reduced, which was confirmed by RT-qPCR (P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0093073