The AmAZI1ng roles of centriolar satellites during development

Given the high conservation of Azi1 among ciliated organisms, the loss-of-function phenotypes from various model organisms, as well as the siRNA results, it was safe to predict that Azi1 would have a central role in mammalian cilia biology in vivo. [...]the authors generated mouse mutants null for Azi1 to test the role of this protein (and centriolar satellites) in mammalian development. [...]this study also highlights the importance of functional follow-up studies of siRNA-based data and cautions against direct extrapolation of transient loss-of-function phenotypes to the genetic in vivo phenotype.