Low pressure radio-frequency oxygen plasma induced oxidation of titanium--surface characteristics and biological effects
This research was designed to investigate the effects of low pressure radio-frequency (RF) oxygen plasma treatment (OPT) on the surface of commercially pure titanium (CP-Ti) and Ti6Al4V. Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed...
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description | This research was designed to investigate the effects of low pressure radio-frequency (RF) oxygen plasma treatment (OPT) on the surface of commercially pure titanium (CP-Ti) and Ti6Al4V. Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed to evaluate the biocompatibility of titanium samples with different lengths of OP treating time.
CP-Ti and Ti6Al4V discs were both classified into 4 groups: untreated, treated with OP generated by using oxygen (99.98%) for 5, 10, and 30 min, respectively. After OPT on CP-Ti and Ti6Al4V samples, scanning probe microscopy, X-ray photoelectron spectrometry (XPS), and contact angle tests were conducted to determine the surface topography, elemental composition and hydrophilicity, respectively. The change of surface morphology was further studied using sputtered titanium on silicon wafers. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and F-actin immunofluorescence stain were performed to investigate the viability and spreading behavior of cultivated MG-63 cells on the samples.
The surface roughness was most prominent after 5 min OPT in both CP-Ti and Ti6Al4V, and the surface morphology of sputtered Ti sharpened after the 5 min treatment. From the XPS results, the intensity of Ti(°), Ti(2+), and Ti(3+) of the samples' surface decreased indicating the oxidation of titanium after OPT. The water contact angles of both CP-Ti and Ti6Al4V were increased after 5 min OPT. The results of MTT assay demonstrated MG-63 cells proliferated best on the 5 min OP treated titanium sample. The F-actin immunofluorescence stain revealed the cultivated cell number of 5 min treated CP-Ti/Ti6Al4V was greater than other groups and most of the cultivated cells were spindle-shaped.
Low pressure RF oxygen plasma modified both the composition and the morphology of titanium samples' surface. The CP-Ti/Ti6Al4V treated with 5 min OPT displayed the roughest surface, sharpest surface profile and best biocompatibility. |
doi_str_mv | 10.1371/journal.pone.0084898 |
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CP-Ti and Ti6Al4V discs were both classified into 4 groups: untreated, treated with OP generated by using oxygen (99.98%) for 5, 10, and 30 min, respectively. After OPT on CP-Ti and Ti6Al4V samples, scanning probe microscopy, X-ray photoelectron spectrometry (XPS), and contact angle tests were conducted to determine the surface topography, elemental composition and hydrophilicity, respectively. The change of surface morphology was further studied using sputtered titanium on silicon wafers. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and F-actin immunofluorescence stain were performed to investigate the viability and spreading behavior of cultivated MG-63 cells on the samples.
The surface roughness was most prominent after 5 min OPT in both CP-Ti and Ti6Al4V, and the surface morphology of sputtered Ti sharpened after the 5 min treatment. From the XPS results, the intensity of Ti(°), Ti(2+), and Ti(3+) of the samples' surface decreased indicating the oxidation of titanium after OPT. The water contact angles of both CP-Ti and Ti6Al4V were increased after 5 min OPT. The results of MTT assay demonstrated MG-63 cells proliferated best on the 5 min OP treated titanium sample. The F-actin immunofluorescence stain revealed the cultivated cell number of 5 min treated CP-Ti/Ti6Al4V was greater than other groups and most of the cultivated cells were spindle-shaped.
Low pressure RF oxygen plasma modified both the composition and the morphology of titanium samples' surface. The CP-Ti/Ti6Al4V treated with 5 min OPT displayed the roughest surface, sharpest surface profile and best biocompatibility.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0084898</identifier><identifier>PMID: 24386433</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actin ; Biocompatibility ; Biological effects ; Biomedical materials ; Cell adhesion & migration ; Cell growth ; Cell Line, Tumor ; Cell morphology ; Cell number ; Cell Proliferation ; Chemical composition ; Contact angle ; Cytology ; Dentistry ; Engineering ; Gene expression ; Humans ; Immunofluorescence ; Insurance claims ; Low pressure ; Materials science ; Materials Testing ; Microscopy ; Morphology ; Nanocomposites ; Nanoparticles ; Oxidation ; Oxidation-Reduction ; Oxygen ; Oxygen - chemistry ; Oxygen plasma ; Photoelectrons ; Pressure ; Pressure effects ; Radio frequency ; Radio Waves ; Scanning probe microscopy ; Spectrometry ; Stains ; Surface Properties ; Surface roughness ; Surgical implants ; Titanium ; Titanium - chemistry ; Titanium alloys ; Titanium base alloys ; Topography ; Transplants & implants ; Ultrasonic testing ; X ray photoelectron spectroscopy</subject><ispartof>PloS one, 2013-12, Vol.8 (12), p.e84898-e84898</ispartof><rights>2013 Tseng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Tseng et al 2013 Tseng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-408c7e6e2bba451ca08cf46204465028883cb1fae53d57a1d97c9d1d3ea5306b3</citedby><cites>FETCH-LOGICAL-c526t-408c7e6e2bba451ca08cf46204465028883cb1fae53d57a1d97c9d1d3ea5306b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873422/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3873422/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24386433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tseng, Wan-Yu</creatorcontrib><creatorcontrib>Hsu, Sheng-Hao</creatorcontrib><creatorcontrib>Huang, Chieh-Hsiun</creatorcontrib><creatorcontrib>Tu, Yu-Chieh</creatorcontrib><creatorcontrib>Tseng, Shao-Chin</creatorcontrib><creatorcontrib>Chen, Hsuen-Li</creatorcontrib><creatorcontrib>Chen, Min-Huey</creatorcontrib><creatorcontrib>Su, Wei-Fang</creatorcontrib><creatorcontrib>Lin, Li-Deh</creatorcontrib><title>Low pressure radio-frequency oxygen plasma induced oxidation of titanium--surface characteristics and biological effects</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This research was designed to investigate the effects of low pressure radio-frequency (RF) oxygen plasma treatment (OPT) on the surface of commercially pure titanium (CP-Ti) and Ti6Al4V. Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed to evaluate the biocompatibility of titanium samples with different lengths of OP treating time.
CP-Ti and Ti6Al4V discs were both classified into 4 groups: untreated, treated with OP generated by using oxygen (99.98%) for 5, 10, and 30 min, respectively. After OPT on CP-Ti and Ti6Al4V samples, scanning probe microscopy, X-ray photoelectron spectrometry (XPS), and contact angle tests were conducted to determine the surface topography, elemental composition and hydrophilicity, respectively. The change of surface morphology was further studied using sputtered titanium on silicon wafers. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and F-actin immunofluorescence stain were performed to investigate the viability and spreading behavior of cultivated MG-63 cells on the samples.
The surface roughness was most prominent after 5 min OPT in both CP-Ti and Ti6Al4V, and the surface morphology of sputtered Ti sharpened after the 5 min treatment. From the XPS results, the intensity of Ti(°), Ti(2+), and Ti(3+) of the samples' surface decreased indicating the oxidation of titanium after OPT. The water contact angles of both CP-Ti and Ti6Al4V were increased after 5 min OPT. The results of MTT assay demonstrated MG-63 cells proliferated best on the 5 min OP treated titanium sample. The F-actin immunofluorescence stain revealed the cultivated cell number of 5 min treated CP-Ti/Ti6Al4V was greater than other groups and most of the cultivated cells were spindle-shaped.
Low pressure RF oxygen plasma modified both the composition and the morphology of titanium samples' surface. The CP-Ti/Ti6Al4V treated with 5 min OPT displayed the roughest surface, sharpest surface profile and best biocompatibility.</description><subject>Actin</subject><subject>Biocompatibility</subject><subject>Biological effects</subject><subject>Biomedical materials</subject><subject>Cell adhesion & migration</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell morphology</subject><subject>Cell number</subject><subject>Cell Proliferation</subject><subject>Chemical composition</subject><subject>Contact angle</subject><subject>Cytology</subject><subject>Dentistry</subject><subject>Engineering</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Insurance claims</subject><subject>Low pressure</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Nanocomposites</subject><subject>Nanoparticles</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxygen</subject><subject>Oxygen - chemistry</subject><subject>Oxygen plasma</subject><subject>Photoelectrons</subject><subject>Pressure</subject><subject>Pressure effects</subject><subject>Radio frequency</subject><subject>Radio Waves</subject><subject>Scanning probe microscopy</subject><subject>Spectrometry</subject><subject>Stains</subject><subject>Surface Properties</subject><subject>Surface roughness</subject><subject>Surgical implants</subject><subject>Titanium</subject><subject>Titanium - chemistry</subject><subject>Titanium alloys</subject><subject>Titanium base alloys</subject><subject>Topography</subject><subject>Transplants & implants</subject><subject>Ultrasonic testing</subject><subject>X ray photoelectron 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pressure radio-frequency oxygen plasma induced oxidation of titanium--surface characteristics and biological effects</title><author>Tseng, Wan-Yu ; Hsu, Sheng-Hao ; Huang, Chieh-Hsiun ; Tu, Yu-Chieh ; Tseng, Shao-Chin ; Chen, Hsuen-Li ; Chen, Min-Huey ; Su, Wei-Fang ; Lin, Li-Deh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-408c7e6e2bba451ca08cf46204465028883cb1fae53d57a1d97c9d1d3ea5306b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Actin</topic><topic>Biocompatibility</topic><topic>Biological effects</topic><topic>Biomedical materials</topic><topic>Cell adhesion & migration</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell morphology</topic><topic>Cell number</topic><topic>Cell Proliferation</topic><topic>Chemical composition</topic><topic>Contact 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induced oxidation of titanium--surface characteristics and biological effects</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-12-26</date><risdate>2013</risdate><volume>8</volume><issue>12</issue><spage>e84898</spage><epage>e84898</epage><pages>e84898-e84898</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This research was designed to investigate the effects of low pressure radio-frequency (RF) oxygen plasma treatment (OPT) on the surface of commercially pure titanium (CP-Ti) and Ti6Al4V. Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed to evaluate the biocompatibility of titanium samples with different lengths of OP treating time.
CP-Ti and Ti6Al4V discs were both classified into 4 groups: untreated, treated with OP generated by using oxygen (99.98%) for 5, 10, and 30 min, respectively. After OPT on CP-Ti and Ti6Al4V samples, scanning probe microscopy, X-ray photoelectron spectrometry (XPS), and contact angle tests were conducted to determine the surface topography, elemental composition and hydrophilicity, respectively. The change of surface morphology was further studied using sputtered titanium on silicon wafers. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and F-actin immunofluorescence stain were performed to investigate the viability and spreading behavior of cultivated MG-63 cells on the samples.
The surface roughness was most prominent after 5 min OPT in both CP-Ti and Ti6Al4V, and the surface morphology of sputtered Ti sharpened after the 5 min treatment. From the XPS results, the intensity of Ti(°), Ti(2+), and Ti(3+) of the samples' surface decreased indicating the oxidation of titanium after OPT. The water contact angles of both CP-Ti and Ti6Al4V were increased after 5 min OPT. The results of MTT assay demonstrated MG-63 cells proliferated best on the 5 min OP treated titanium sample. The F-actin immunofluorescence stain revealed the cultivated cell number of 5 min treated CP-Ti/Ti6Al4V was greater than other groups and most of the cultivated cells were spindle-shaped.
Low pressure RF oxygen plasma modified both the composition and the morphology of titanium samples' surface. The CP-Ti/Ti6Al4V treated with 5 min OPT displayed the roughest surface, sharpest surface profile and best biocompatibility.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24386433</pmid><doi>10.1371/journal.pone.0084898</doi><oa>free_for_read</oa></addata></record> |
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language | eng |
recordid | cdi_plos_journals_1474889005 |
source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
subjects | Actin Biocompatibility Biological effects Biomedical materials Cell adhesion & migration Cell growth Cell Line, Tumor Cell morphology Cell number Cell Proliferation Chemical composition Contact angle Cytology Dentistry Engineering Gene expression Humans Immunofluorescence Insurance claims Low pressure Materials science Materials Testing Microscopy Morphology Nanocomposites Nanoparticles Oxidation Oxidation-Reduction Oxygen Oxygen - chemistry Oxygen plasma Photoelectrons Pressure Pressure effects Radio frequency Radio Waves Scanning probe microscopy Spectrometry Stains Surface Properties Surface roughness Surgical implants Titanium Titanium - chemistry Titanium alloys Titanium base alloys Topography Transplants & implants Ultrasonic testing X ray photoelectron spectroscopy |
title | Low pressure radio-frequency oxygen plasma induced oxidation of titanium--surface characteristics and biological effects |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T22%3A49%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Low%20pressure%20radio-frequency%20oxygen%20plasma%20induced%20oxidation%20of%20titanium--surface%20characteristics%20and%20biological%20effects&rft.jtitle=PloS%20one&rft.au=Tseng,%20Wan-Yu&rft.date=2013-12-26&rft.volume=8&rft.issue=12&rft.spage=e84898&rft.epage=e84898&rft.pages=e84898-e84898&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0084898&rft_dat=%3Cproquest_plos_%3E3174559491%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1474889005&rft_id=info:pmid/24386433&rft_doaj_id=oai_doaj_org_article_4c5f9612db62451a960c799c0753232c&rfr_iscdi=true |