Low pressure radio-frequency oxygen plasma induced oxidation of titanium--surface characteristics and biological effects

This research was designed to investigate the effects of low pressure radio-frequency (RF) oxygen plasma treatment (OPT) on the surface of commercially pure titanium (CP-Ti) and Ti6Al4V. Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed...

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Veröffentlicht in:PloS one 2013-12, Vol.8 (12), p.e84898-e84898
Hauptverfasser: Tseng, Wan-Yu, Hsu, Sheng-Hao, Huang, Chieh-Hsiun, Tu, Yu-Chieh, Tseng, Shao-Chin, Chen, Hsuen-Li, Chen, Min-Huey, Su, Wei-Fang, Lin, Li-Deh
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container_issue 12
container_start_page e84898
container_title PloS one
container_volume 8
creator Tseng, Wan-Yu
Hsu, Sheng-Hao
Huang, Chieh-Hsiun
Tu, Yu-Chieh
Tseng, Shao-Chin
Chen, Hsuen-Li
Chen, Min-Huey
Su, Wei-Fang
Lin, Li-Deh
description This research was designed to investigate the effects of low pressure radio-frequency (RF) oxygen plasma treatment (OPT) on the surface of commercially pure titanium (CP-Ti) and Ti6Al4V. Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed to evaluate the biocompatibility of titanium samples with different lengths of OP treating time. CP-Ti and Ti6Al4V discs were both classified into 4 groups: untreated, treated with OP generated by using oxygen (99.98%) for 5, 10, and 30 min, respectively. After OPT on CP-Ti and Ti6Al4V samples, scanning probe microscopy, X-ray photoelectron spectrometry (XPS), and contact angle tests were conducted to determine the surface topography, elemental composition and hydrophilicity, respectively. The change of surface morphology was further studied using sputtered titanium on silicon wafers. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and F-actin immunofluorescence stain were performed to investigate the viability and spreading behavior of cultivated MG-63 cells on the samples. The surface roughness was most prominent after 5 min OPT in both CP-Ti and Ti6Al4V, and the surface morphology of sputtered Ti sharpened after the 5 min treatment. From the XPS results, the intensity of Ti(°), Ti(2+), and Ti(3+) of the samples' surface decreased indicating the oxidation of titanium after OPT. The water contact angles of both CP-Ti and Ti6Al4V were increased after 5 min OPT. The results of MTT assay demonstrated MG-63 cells proliferated best on the 5 min OP treated titanium sample. The F-actin immunofluorescence stain revealed the cultivated cell number of 5 min treated CP-Ti/Ti6Al4V was greater than other groups and most of the cultivated cells were spindle-shaped. Low pressure RF oxygen plasma modified both the composition and the morphology of titanium samples' surface. The CP-Ti/Ti6Al4V treated with 5 min OPT displayed the roughest surface, sharpest surface profile and best biocompatibility.
doi_str_mv 10.1371/journal.pone.0084898
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Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed to evaluate the biocompatibility of titanium samples with different lengths of OP treating time. CP-Ti and Ti6Al4V discs were both classified into 4 groups: untreated, treated with OP generated by using oxygen (99.98%) for 5, 10, and 30 min, respectively. After OPT on CP-Ti and Ti6Al4V samples, scanning probe microscopy, X-ray photoelectron spectrometry (XPS), and contact angle tests were conducted to determine the surface topography, elemental composition and hydrophilicity, respectively. The change of surface morphology was further studied using sputtered titanium on silicon wafers. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and F-actin immunofluorescence stain were performed to investigate the viability and spreading behavior of cultivated MG-63 cells on the samples. The surface roughness was most prominent after 5 min OPT in both CP-Ti and Ti6Al4V, and the surface morphology of sputtered Ti sharpened after the 5 min treatment. From the XPS results, the intensity of Ti(°), Ti(2+), and Ti(3+) of the samples' surface decreased indicating the oxidation of titanium after OPT. The water contact angles of both CP-Ti and Ti6Al4V were increased after 5 min OPT. The results of MTT assay demonstrated MG-63 cells proliferated best on the 5 min OP treated titanium sample. The F-actin immunofluorescence stain revealed the cultivated cell number of 5 min treated CP-Ti/Ti6Al4V was greater than other groups and most of the cultivated cells were spindle-shaped. Low pressure RF oxygen plasma modified both the composition and the morphology of titanium samples' surface. 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Surface topography, elemental composition, water contact angle, cell viability, and cell morphology were surveyed to evaluate the biocompatibility of titanium samples with different lengths of OP treating time. CP-Ti and Ti6Al4V discs were both classified into 4 groups: untreated, treated with OP generated by using oxygen (99.98%) for 5, 10, and 30 min, respectively. After OPT on CP-Ti and Ti6Al4V samples, scanning probe microscopy, X-ray photoelectron spectrometry (XPS), and contact angle tests were conducted to determine the surface topography, elemental composition and hydrophilicity, respectively. The change of surface morphology was further studied using sputtered titanium on silicon wafers. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and F-actin immunofluorescence stain were performed to investigate the viability and spreading behavior of cultivated MG-63 cells on the samples. The surface roughness was most prominent after 5 min OPT in both CP-Ti and Ti6Al4V, and the surface morphology of sputtered Ti sharpened after the 5 min treatment. From the XPS results, the intensity of Ti(°), Ti(2+), and Ti(3+) of the samples' surface decreased indicating the oxidation of titanium after OPT. The water contact angles of both CP-Ti and Ti6Al4V were increased after 5 min OPT. The results of MTT assay demonstrated MG-63 cells proliferated best on the 5 min OP treated titanium sample. The F-actin immunofluorescence stain revealed the cultivated cell number of 5 min treated CP-Ti/Ti6Al4V was greater than other groups and most of the cultivated cells were spindle-shaped. Low pressure RF oxygen plasma modified both the composition and the morphology of titanium samples' surface. The CP-Ti/Ti6Al4V treated with 5 min OPT displayed the roughest surface, sharpest surface profile and best biocompatibility.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24386433</pmid><doi>10.1371/journal.pone.0084898</doi><oa>free_for_read</oa></addata></record>
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issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1474889005
source MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS)
subjects Actin
Biocompatibility
Biological effects
Biomedical materials
Cell adhesion & migration
Cell growth
Cell Line, Tumor
Cell morphology
Cell number
Cell Proliferation
Chemical composition
Contact angle
Cytology
Dentistry
Engineering
Gene expression
Humans
Immunofluorescence
Insurance claims
Low pressure
Materials science
Materials Testing
Microscopy
Morphology
Nanocomposites
Nanoparticles
Oxidation
Oxidation-Reduction
Oxygen
Oxygen - chemistry
Oxygen plasma
Photoelectrons
Pressure
Pressure effects
Radio frequency
Radio Waves
Scanning probe microscopy
Spectrometry
Stains
Surface Properties
Surface roughness
Surgical implants
Titanium
Titanium - chemistry
Titanium alloys
Titanium base alloys
Topography
Transplants & implants
Ultrasonic testing
X ray photoelectron spectroscopy
title Low pressure radio-frequency oxygen plasma induced oxidation of titanium--surface characteristics and biological effects
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