Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro

Cathepsin K, a lysosomal cysteine protease, is expressed in the tumor microenvironment (TME) of skin carcinoma, but nothing is known about cathepsin K in oral tongue squamous cell carcinoma (OTSCC). Our aim was to describe the expression of cathepsin K in invasive OTSCC in vitro and in a series of c...

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Veröffentlicht in:	PloS one 2013-08, Vol.8 (8), p.e70925-e70925
Hauptverfasser:	Bitu, Carolina C, Kauppila, Joonas H, Bufalino, Andréia, Nurmenniemi, Sini, Teppo, Susanna, Keinänen, Meeri, Vilen, Suvi-Tuuli, Lehenkari, Petri, Nyberg, Pia, Coletta, Ricardo D, Salo, Tuula
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Analysis Arthritis Biology Blotting, Western Breast cancer Cancer Cancer metastasis Carcinoma, Squamous Cell - enzymology Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - pathology Cathepsin K Cathepsin K - antagonists & inhibitors Cathepsin K - genetics Cathepsin K - metabolism Cathepsins Cell Line, Tumor Cells, Cultured Cysteine Cysteine proteinase Cysteine Proteinase Inhibitors - pharmacology Cystine Dentistry Development and progression Disease Progression Extracellular matrix Female Fibroblasts Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Homeostasis Hospitals Humans Immunohistochemistry In vitro methods and tests Invasiveness Leucine - analogs & derivatives Leucine - pharmacology Lymph nodes Lymphatic Metastasis Male Medicine Metastases Middle Aged Neoplasm Invasiveness Neoplasm Recurrence, Local Oral diseases Organ Culture Techniques Osteoporosis Prognosis Prostate cancer Protease Protease inhibitors Reverse Transcriptase Polymerase Chain Reaction RNA Interference Skin Skin carcinoma Squamous cell carcinoma Three dimensional models Tongue Tongue Neoplasms - enzymology Tongue Neoplasms - genetics Tongue Neoplasms - pathology Tumor Microenvironment - genetics Tumors
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Zusammenfassung:	Cathepsin K, a lysosomal cysteine protease, is expressed in the tumor microenvironment (TME) of skin carcinoma, but nothing is known about cathepsin K in oral tongue squamous cell carcinoma (OTSCC). Our aim was to describe the expression of cathepsin K in invasive OTSCC in vitro and in a series of clinical cancer specimens. OTSCC invasion in vitro was studied using invasive HSC-3 tongue carcinoma cells in 3D organotypic models. In total, 121 mobile tongue OTSCCs and 10 lymph node metastases were analyzed for cathepsin K expression. The association between cathepsin K expression and clinicopathological factors was evaluated. Cysteine protease inhibitor E64 and cathepsin K silencing significantly (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0070925