Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae
Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the...
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| Veröffentlicht in: | PloS one 2013-06, Vol.8 (6), p.e66358-e66358 |
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| creator | Miyoshi-Akiyama, Tohru Hayakawa, Kayoko Ohmagari, Norio Shimojima, Masahiro Kirikae, Teruo |
| description | Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study. |
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Y.</contributor><creatorcontrib>Miyoshi-Akiyama, Tohru ; Hayakawa, Kayoko ; Ohmagari, Norio ; Shimojima, Masahiro ; Kirikae, Teruo ; Woo, Patrick C. Y.</creatorcontrib><description>Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0066358</identifier><identifier>PMID: 23776664</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Bacteria ; Beta lactamases ; Bioinformatics ; Biology ; Clinical isolates ; Deoxyribonucleic acid ; Disease control ; DNA ; Drug resistance ; Enterobacter ; Enterobacter cloacae ; Enterobacter cloacae - genetics ; Epidemics ; Epidemiology ; Genetics ; Genomes ; Health aspects ; Hospitals ; Infection ; Infections ; Infectious diseases ; Laboratories ; Medicine ; Methods ; Multilocus sequence typing ; Multilocus Sequence Typing - methods ; Mutation ; Neonates ; Outbreaks ; Performance evaluation ; Phylogenetics ; Population growth ; Sepsis ; Sheep ; Statistical analysis ; Surgery ; Surgical site infections ; Urinary tract infections</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e66358-e66358</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Miyoshi-Akiyama et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Miyoshi-Akiyama et al 2013 Miyoshi-Akiyama et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-f884e91a5747830c30bb980a245b5970b0ec150c0f819a57cfff2e56111b59d83</citedby><cites>FETCH-LOGICAL-c692t-f884e91a5747830c30bb980a245b5970b0ec150c0f819a57cfff2e56111b59d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679064/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679064/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23776664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Woo, Patrick C. Y.</contributor><creatorcontrib>Miyoshi-Akiyama, Tohru</creatorcontrib><creatorcontrib>Hayakawa, Kayoko</creatorcontrib><creatorcontrib>Ohmagari, Norio</creatorcontrib><creatorcontrib>Shimojima, Masahiro</creatorcontrib><creatorcontrib>Kirikae, Teruo</creatorcontrib><title>Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. 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methods</subject><subject>Mutation</subject><subject>Neonates</subject><subject>Outbreaks</subject><subject>Performance evaluation</subject><subject>Phylogenetics</subject><subject>Population growth</subject><subject>Sepsis</subject><subject>Sheep</subject><subject>Statistical analysis</subject><subject>Surgery</subject><subject>Surgical site infections</subject><subject>Urinary tract 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Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-11</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e66358</spage><epage>e66358</epage><pages>e66358-e66358</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. 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| subjects | Bacteria Beta lactamases Bioinformatics Biology Clinical isolates Deoxyribonucleic acid Disease control DNA Drug resistance Enterobacter Enterobacter cloacae Enterobacter cloacae - genetics Epidemics Epidemiology Genetics Genomes Health aspects Hospitals Infection Infections Infectious diseases Laboratories Medicine Methods Multilocus sequence typing Multilocus Sequence Typing - methods Mutation Neonates Outbreaks Performance evaluation Phylogenetics Population growth Sepsis Sheep Statistical analysis Surgery Surgical site infections Urinary tract infections |
| title | Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae |
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