High myeloperoxidase positive cell infiltration in colorectal cancer is an independent favorable prognostic factor

Colorectal cancer (CRC) infiltration by adaptive immune system cells correlates with favorable prognosis. The role of the innate immune system is still debated. Here we addressed the prognostic impact of CRC infiltration by neutrophil granulocytes (NG). A TMA including healthy mucosa and clinically...

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Veröffentlicht in:	PloS one 2013-05, Vol.8 (5), p.e64814-e64814
Hauptverfasser:	Droeser, Raoul A, Hirt, Christian, Eppenberger-Castori, Serenella, Zlobec, Inti, Viehl, Carsten T, Frey, Daniel M, Nebiker, Christian A, Rosso, Raffaele, Zuber, Markus, Amicarella, Francesca, Iezzi, Giandomenica, Sconocchia, Giuseppe, Heberer, Michael, Lugli, Alessandro, Tornillo, Luigi, Oertli, Daniel, Terracciano, Luigi, Spagnoli, Giulio C
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptive systems Adult Aged Aged, 80 and over Antibodies Antigens Antigens, CD - immunology Antigens, CD - metabolism Antigens, Differentiation, Myelomonocytic - immunology Antigens, Differentiation, Myelomonocytic - metabolism Biology Cancer Cell Adhesion Molecules - immunology Cell Adhesion Molecules - metabolism Cell death Cell survival Cell suspensions Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - immunology Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Cytokines Cytometry Female Flow Cytometry Fucosyltransferases - immunology Fucosyltransferases - metabolism Gene expression GPI-Linked Proteins - immunology GPI-Linked Proteins - metabolism Granulocytes Hospital administration Humans Immune system Immunohistochemistry Infiltration Innate immunity Leukocytes (granulocytic) Lewis X Antigen - immunology Lewis X Antigen - metabolism Male Medical prognosis Medicine Metastases Metastasis Microsatellite instability Microsatellites Middle Aged Mucosa Multivariate Analysis Neoplasm Grading Neoplasm Staging Neutrophils Neutrophils - immunology Neutrophils - metabolism Neutrophils - pathology Oxidative stress Pathology Peroxidase Peroxidase - immunology Peroxidase - metabolism Prognosis Receptors, IgG - immunology Receptors, IgG - metabolism Splitting Stability Surgery Survival Survival Analysis Training Tumors
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Zusammenfassung:	Colorectal cancer (CRC) infiltration by adaptive immune system cells correlates with favorable prognosis. The role of the innate immune system is still debated. Here we addressed the prognostic impact of CRC infiltration by neutrophil granulocytes (NG). A TMA including healthy mucosa and clinically annotated CRC specimens (n = 1491) was stained with MPO and CD15 specific antibodies. MPO+ and CD15+ positive immune cells were counted by three independent observers. Phenotypic profiles of CRC infiltrating MPO+ and CD15+ cells were validated by flow cytometry on cell suspensions derived from enzymatically digested surgical specimens. Survival analysis was performed by splitting randomized data in training and validation subsets. MPO+ and CD15+ cell infiltration were significantly correlated (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0064814