Exopolymer diversity and the role of levan in Bacillus subtilis biofilms

Exopolymeric substances (EPS) are important for biofilm formation and their chemical composition may influence biofilm properties. To explore these relationships the chemical composition of EPS from Bacillus subtilis NCIB 3610 biofilms grown in sucrose-rich (SYM) and sucrose-poor (MSgg and Czapek) m...

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Veröffentlicht in:	PloS one 2013-04, Vol.8 (4), p.e62044
Hauptverfasser:	Dogsa, Iztok, Brloznik, Mojca, Stopar, David, Mandic-Mulec, Ines
Format:	Artikel
Sprache:	eng
Schlagworte:	Addition polymerization Bacillus Bacillus subtilis Bacillus subtilis - drug effects Bacillus subtilis - genetics Bacillus subtilis - growth & development Bacillus subtilis - physiology Bacterial Proteins - isolation & purification Biofilms Biofilms - drug effects Biofilms - growth & development Biology Biomass Biopesticides Biopolymers - metabolism Chemical composition Chromatography, Gel Control stability Culture Media - pharmacology Defects Deoxyribonucleic acid Dietary supplements DNA Enzymes Extracellular matrix Floating structures Food science Fructans - metabolism Gene Expression Regulation, Bacterial - drug effects Gram-positive bacteria Levan Levans Media Mutants Nucleic Acids - isolation & purification Operon - genetics Phenotype Polymers Polysaccharides Polysaccharides, Bacterial - isolation & purification Proteins Saccharides Sucrose Sucrose - pharmacology Sugar Time Factors
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Zusammenfassung:	Exopolymeric substances (EPS) are important for biofilm formation and their chemical composition may influence biofilm properties. To explore these relationships the chemical composition of EPS from Bacillus subtilis NCIB 3610 biofilms grown in sucrose-rich (SYM) and sucrose-poor (MSgg and Czapek) media was studied. We observed marked differences in composition of EPS polymers isolated from all three biofilms or from spent media below the biofilms. The polysaccharide levan dominated the EPS of SYM grown biofilms, while EPS from biofilms grown in sucrose-poor media contained significant amounts of proteins and DNA in addition to polysaccharides. The EPS polymers differed also in size with very large polymers (Mw>2000 kDa) found only in biofilms, while small polymers (Mw
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0062044