A novel immunofluorescent computed tomography (ICT) method to localise and quantify multiple antigens in large tissue volumes at high resolution

A novel immunofluorescent computed tomography (ICT) method to localise and quantify multiple antigens in large tissue volumes at high resolution

Current immunofluorescence protocols are limited as they do not provide reliable antibody staining within large tissue volumes (mm(3)) and cannot localise and quantify multiple antigens or cell populations in the same tissue at high resolution. To address this limitation, we have developed an approa...

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Veröffentlicht in:PloS one 2012-12, Vol.7 (12), p.e53245-e53245
Hauptverfasser: Parfitt, Geraint J, Xie, Yilu, Reid, Korey M, Dervillez, Xavier, Brown, Donald J, Jester, James V
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibodies
Antigenicity
Antigens
Biology
Biomolecules
CAT scans
Cell density
Cell Proliferation
Collagen
Collagen - metabolism
Computation
Computed tomography
Cytokeratin
Embedding
Evaluation
Eyelid
Eyelids - diagnostic imaging
Eyelids - metabolism
Fluorescence
Fluorescent Antibody Technique - methods
Genetic engineering
High resolution
Image Processing, Computer-Assisted - methods
Imaging, Three-Dimensional - methods
Immunofluorescence
Information technology
Iterative methods
Keratin
Labeling
Labelling
Light
Medical imaging
Medicine
Meibomian Glands - diagnostic imaging
Meibomian Glands - metabolism
Methods
Methyl methacrylate
Mice
Microscopy
Nuclei
Nuclei (cytology)
Optics
Polymethyl methacrylate
Proteins
Protocol (computers)
Quantitative analysis
Reconstruction
Structure-function relationships
Tomography
Tomography, X-Ray Computed - methods
Transgenic
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Zusammenfassung:Current immunofluorescence protocols are limited as they do not provide reliable antibody staining within large tissue volumes (mm(3)) and cannot localise and quantify multiple antigens or cell populations in the same tissue at high resolution. To address this limitation, we have developed an approach to three-dimensionally visualise large tissue volumes (mm(3)) at high resolution (
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0053245