Blunted sodium excretion in response to a saline load in 5 year old female sheep following fetal uninephrectomy
Previously, we have shown that fetal uninephrectomy (uni-x) causes hypertension in female sheep by 2 years of age. Whilst the hypertension was not exacerbated by 5 years of age, these uni-x sheep had greater reductions in renal blood flow (RBF). To further explore these early indications of a declin...
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| description | Previously, we have shown that fetal uninephrectomy (uni-x) causes hypertension in female sheep by 2 years of age. Whilst the hypertension was not exacerbated by 5 years of age, these uni-x sheep had greater reductions in renal blood flow (RBF). To further explore these early indications of a decline in renal function, we investigated the renal response to a saline load (25 ml/kg/40 min) in 5-year old female uni-x and sham sheep. Basal mean arterial pressure was ∼15 mmHg greater (P(Group) |
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Whilst the hypertension was not exacerbated by 5 years of age, these uni-x sheep had greater reductions in renal blood flow (RBF). To further explore these early indications of a decline in renal function, we investigated the renal response to a saline load (25 ml/kg/40 min) in 5-year old female uni-x and sham sheep. Basal mean arterial pressure was ∼15 mmHg greater (P(Group)<0.001), and sodium excretion (∼50%), glomerular filtration rate (∼30%, GFR) and RBF (∼40%) were all significantly lower (P(Group)<0.01) in uni-x compared to sham animals. In response to saline loading, sodium excretion increased significantly in both groups (P(Time)<0.001), however this response was blunted in uni-x sheep (P(GroupxTime)<0.01). This was accompanied with an attenuated increase in GFR and fractional sodium excretion (both P(GroupxTime)<0.05), and reduced activation of the renin-angiotensin system (both P<0.05), as compared to the sham group. The reduction in sodium excretion was associated with up-regulations in the renal gene expression of NHE3 and Na(+)/K(+) ATPase α and β subunits in the kidney cortex of the uni-x compared to the sham animals (P<0.05). Notably, neither group completely excreted the saline load within the recovery period, but the uni-x retained a higher percentage of the total volume (uni-x: 48±7%; sham: 22±9%, P<0.05). In conclusion, a reduced ability to efficiently regulate extracellular fluid homeostasis is evident in female sheep at 5 years of age, which was exacerbated in animals born with a congenital nephron deficit. Whilst there was no overt exacerbation of hypertension and renal insufficiency with age in the uni-x sheep, these animals may be more vulnerable to secondary renal insults.]]></description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0047528</identifier><identifier>PMID: 23077628</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Angiotensin ; Angiotensins ; Animals ; Arterial Pressure ; Biology ; Blood flow ; Blood pressure ; Cardiovascular disease ; Congenital diseases ; Development and progression ; Endowment ; Excretion ; Female ; Females ; Fetuses ; Gene expression ; Gene Expression - drug effects ; Genetic aspects ; Glomerular filtration rate ; Glomerular Filtration Rate - drug effects ; Homeostasis ; Hypertension ; Hypertension - chemically induced ; Kidney diseases ; Males ; Medicine ; Na+/K+-exchanging ATPase ; Nephrectomy - adverse effects ; Ovis aries ; Physiology ; Psychological aspects ; Renal Circulation - drug effects ; Renal cortex ; Renal function ; Renal insufficiency ; Renin ; Risk factors ; Rodents ; Sheep ; Sodium ; Sodium - analysis ; Sodium Chloride - administration & dosage ; Sodium-Potassium-Exchanging ATPase - metabolism ; Surgery ; Veterinary Science</subject><ispartof>PloS one, 2012-10, Vol.7 (10), p.e47528</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Lankadeva et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Lankadeva et al 2012 Lankadeva et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-c1dffdc1c6f3c8a6d6c3bef212239a9dc8b9cda7cc91b7ead6f35bd5758a29243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471853/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471853/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23077628$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sen, Utpal</contributor><creatorcontrib>Lankadeva, Yugeesh R</creatorcontrib><creatorcontrib>Singh, Reetu R</creatorcontrib><creatorcontrib>Hilliard, Lucinda M</creatorcontrib><creatorcontrib>Moritz, Karen M</creatorcontrib><creatorcontrib>Denton, Kate M</creatorcontrib><title>Blunted sodium excretion in response to a saline load in 5 year old female sheep following fetal uninephrectomy</title><title>PloS one</title><addtitle>PLoS One</addtitle><description><![CDATA[Previously, we have shown that fetal uninephrectomy (uni-x) causes hypertension in female sheep by 2 years of age. Whilst the hypertension was not exacerbated by 5 years of age, these uni-x sheep had greater reductions in renal blood flow (RBF). To further explore these early indications of a decline in renal function, we investigated the renal response to a saline load (25 ml/kg/40 min) in 5-year old female uni-x and sham sheep. Basal mean arterial pressure was ∼15 mmHg greater (P(Group)<0.001), and sodium excretion (∼50%), glomerular filtration rate (∼30%, GFR) and RBF (∼40%) were all significantly lower (P(Group)<0.01) in uni-x compared to sham animals. In response to saline loading, sodium excretion increased significantly in both groups (P(Time)<0.001), however this response was blunted in uni-x sheep (P(GroupxTime)<0.01). This was accompanied with an attenuated increase in GFR and fractional sodium excretion (both P(GroupxTime)<0.05), and reduced activation of the renin-angiotensin system (both P<0.05), as compared to the sham group. The reduction in sodium excretion was associated with up-regulations in the renal gene expression of NHE3 and Na(+)/K(+) ATPase α and β subunits in the kidney cortex of the uni-x compared to the sham animals (P<0.05). Notably, neither group completely excreted the saline load within the recovery period, but the uni-x retained a higher percentage of the total volume (uni-x: 48±7%; sham: 22±9%, P<0.05). In conclusion, a reduced ability to efficiently regulate extracellular fluid homeostasis is evident in female sheep at 5 years of age, which was exacerbated in animals born with a congenital nephron deficit. Whilst there was no overt exacerbation of hypertension and renal insufficiency with age in the uni-x sheep, these animals may be more vulnerable to secondary renal insults.]]></description><subject>Age</subject><subject>Angiotensin</subject><subject>Angiotensins</subject><subject>Animals</subject><subject>Arterial Pressure</subject><subject>Biology</subject><subject>Blood flow</subject><subject>Blood pressure</subject><subject>Cardiovascular disease</subject><subject>Congenital diseases</subject><subject>Development and progression</subject><subject>Endowment</subject><subject>Excretion</subject><subject>Female</subject><subject>Females</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Genetic aspects</subject><subject>Glomerular filtration rate</subject><subject>Glomerular Filtration Rate - drug effects</subject><subject>Homeostasis</subject><subject>Hypertension</subject><subject>Hypertension - chemically induced</subject><subject>Kidney diseases</subject><subject>Males</subject><subject>Medicine</subject><subject>Na+/K+-exchanging ATPase</subject><subject>Nephrectomy - adverse effects</subject><subject>Ovis aries</subject><subject>Physiology</subject><subject>Psychological aspects</subject><subject>Renal Circulation - drug effects</subject><subject>Renal cortex</subject><subject>Renal function</subject><subject>Renal insufficiency</subject><subject>Renin</subject><subject>Risk factors</subject><subject>Rodents</subject><subject>Sheep</subject><subject>Sodium</subject><subject>Sodium - analysis</subject><subject>Sodium Chloride - administration & dosage</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Surgery</subject><subject>Veterinary Science</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12P1CAUhhujcdfVf2CUxMToxYwFSmlvTNaNH5Nssolft4TC6QwTWmaB6s6_lzrdzdTsheECODzvCxw4WfYc50tMOX63dYPvpV3uXA_LPC84I9WD7BTXlCxKktOHR-OT7EkI2zxntCrLx9kJoTnnJalOM_fBDn0EjYLTZugQ3CgP0bgemR55CMk9AIoOSRSkNT0g66QeFxnag_TIWY1a6KQFFDYAO9Q6a91v069TOEqLhj6pdhsPKrpu_zR71Eob4NnUn2U_Pn38fvFlcXn1eXVxfrlQnFVxobBuW62wKluqKlnqUtEGWoIJobWstaqaWmnJlapxw0HqxLFGsySWpCYFPcteHnx31gUx5SoITEnJypwXPBGrA6Gd3IqdN530e-GkEX8Dzq-F9NEoC4KpWhZtym_T8AKnCWa8LnhbEMJyIsvk9X7abWg60Ar66KWdmc5XerMRa_dL0ILjitFk8GYy8O56gBBFZ4ICa2UPbkjnxpjWVV7zEX31D3r_7SZqnV5GmL51aV81morzoq4I47wgiVreQ6WmoTMqfazWpPhM8HYmSEyEm7iWQwhi9e3r_7NXP-fs6yN2A9LGTXB2GH9imIPFAVTeheChvUsyzsVYF7fZEGNdiKkukuzF8QPdiW4Lgf4BE6UJkg</recordid><startdate>20121015</startdate><enddate>20121015</enddate><creator>Lankadeva, Yugeesh R</creator><creator>Singh, Reetu R</creator><creator>Hilliard, Lucinda M</creator><creator>Moritz, Karen M</creator><creator>Denton, Kate M</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20121015</creationdate><title>Blunted sodium excretion in response to a saline load in 5 year old female sheep following fetal uninephrectomy</title><author>Lankadeva, Yugeesh R ; Singh, Reetu R ; Hilliard, Lucinda M ; Moritz, Karen M ; Denton, Kate M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-c1dffdc1c6f3c8a6d6c3bef212239a9dc8b9cda7cc91b7ead6f35bd5758a29243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Age</topic><topic>Angiotensin</topic><topic>Angiotensins</topic><topic>Animals</topic><topic>Arterial Pressure</topic><topic>Biology</topic><topic>Blood flow</topic><topic>Blood pressure</topic><topic>Cardiovascular disease</topic><topic>Congenital diseases</topic><topic>Development and progression</topic><topic>Endowment</topic><topic>Excretion</topic><topic>Female</topic><topic>Females</topic><topic>Fetuses</topic><topic>Gene expression</topic><topic>Gene Expression - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lankadeva, Yugeesh R</au><au>Singh, Reetu R</au><au>Hilliard, Lucinda M</au><au>Moritz, Karen M</au><au>Denton, Kate M</au><au>Sen, Utpal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Blunted sodium excretion in response to a saline load in 5 year old female sheep following fetal uninephrectomy</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-10-15</date><risdate>2012</risdate><volume>7</volume><issue>10</issue><spage>e47528</spage><pages>e47528-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract><![CDATA[Previously, we have shown that fetal uninephrectomy (uni-x) causes hypertension in female sheep by 2 years of age. Whilst the hypertension was not exacerbated by 5 years of age, these uni-x sheep had greater reductions in renal blood flow (RBF). To further explore these early indications of a decline in renal function, we investigated the renal response to a saline load (25 ml/kg/40 min) in 5-year old female uni-x and sham sheep. Basal mean arterial pressure was ∼15 mmHg greater (P(Group)<0.001), and sodium excretion (∼50%), glomerular filtration rate (∼30%, GFR) and RBF (∼40%) were all significantly lower (P(Group)<0.01) in uni-x compared to sham animals. In response to saline loading, sodium excretion increased significantly in both groups (P(Time)<0.001), however this response was blunted in uni-x sheep (P(GroupxTime)<0.01). This was accompanied with an attenuated increase in GFR and fractional sodium excretion (both P(GroupxTime)<0.05), and reduced activation of the renin-angiotensin system (both P<0.05), as compared to the sham group. The reduction in sodium excretion was associated with up-regulations in the renal gene expression of NHE3 and Na(+)/K(+) ATPase α and β subunits in the kidney cortex of the uni-x compared to the sham animals (P<0.05). Notably, neither group completely excreted the saline load within the recovery period, but the uni-x retained a higher percentage of the total volume (uni-x: 48±7%; sham: 22±9%, P<0.05). In conclusion, a reduced ability to efficiently regulate extracellular fluid homeostasis is evident in female sheep at 5 years of age, which was exacerbated in animals born with a congenital nephron deficit. Whilst there was no overt exacerbation of hypertension and renal insufficiency with age in the uni-x sheep, these animals may be more vulnerable to secondary renal insults.]]></abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23077628</pmid><doi>10.1371/journal.pone.0047528</doi><tpages>e47528</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Age Angiotensin Angiotensins Animals Arterial Pressure Biology Blood flow Blood pressure Cardiovascular disease Congenital diseases Development and progression Endowment Excretion Female Females Fetuses Gene expression Gene Expression - drug effects Genetic aspects Glomerular filtration rate Glomerular Filtration Rate - drug effects Homeostasis Hypertension Hypertension - chemically induced Kidney diseases Males Medicine Na+/K+-exchanging ATPase Nephrectomy - adverse effects Ovis aries Physiology Psychological aspects Renal Circulation - drug effects Renal cortex Renal function Renal insufficiency Renin Risk factors Rodents Sheep Sodium Sodium - analysis Sodium Chloride - administration & dosage Sodium-Potassium-Exchanging ATPase - metabolism Surgery Veterinary Science |
| title | Blunted sodium excretion in response to a saline load in 5 year old female sheep following fetal uninephrectomy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T16%3A29%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Blunted%20sodium%20excretion%20in%20response%20to%20a%20saline%20load%20in%205%20year%20old%20female%20sheep%20following%20fetal%20uninephrectomy&rft.jtitle=PloS%20one&rft.au=Lankadeva,%20Yugeesh%20R&rft.date=2012-10-15&rft.volume=7&rft.issue=10&rft.spage=e47528&rft.pages=e47528-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0047528&rft_dat=%3Cgale_plos_%3EA498257742%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1326560747&rft_id=info:pmid/23077628&rft_galeid=A498257742&rft_doaj_id=oai_doaj_org_article_5c9a4f528bb741c9a157947f422502a6&rfr_iscdi=true |