Clinical utility of insulin-like growth factor 1 and 2; determination by high resolution mass spectrometry
Measurement of insulin-like growth factor-1 (IGF-I) has utility for the diagnosis and management of growth disorders, but inter-assay comparison of results has been complicated by a multitude of reference standards, antibodies, detection methods, and pre-analytical preparation strategies. We develop...
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Veröffentlicht in: | PloS one 2012-09, Vol.7 (9), p.e43457-e43457 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Measurement of insulin-like growth factor-1 (IGF-I) has utility for the diagnosis and management of growth disorders, but inter-assay comparison of results has been complicated by a multitude of reference standards, antibodies, detection methods, and pre-analytical preparation strategies. We developed a quantitative LC-MS method for intact IGF-I, which has advantages in throughput and complexity when compared to mass spectrometric approaches that rely on stable isotope dilution analysis of tryptic peptides. Since the method makes use of full-scan data, the assay was easily extended to provide quantitative measurement of IGF-II using the same assay protocol. The validated LC-MS assay for IGF-I and IGF-II provides accurate results across the pediatric and adult reference range and is suitable for clinical use. |
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ISSN: | 1932-6203 1932-6203 |
DOI: | 10.1371/journal.pone.0043457 |