StarD7 knockdown modulates ABCG2 expression, cell migration, proliferation, and differentiation of human choriocarcinoma JEG-3 cells

StAR-related lipid transfer domain containing 7 (StarD7) is a member of the START-domain protein family whose function still remains unclear. Our data from an explorative microarray assay performed with mRNAs from StarD7 siRNA-transfected JEG-3 cells indicated that ABCG2 (ATP-binding cassette sub-fa...

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Veröffentlicht in:	PloS one 2012-08, Vol.7 (8), p.e44152-e44152
Hauptverfasser:	Flores-Martín, Jésica, Rena, Viviana, Márquez, Sebastián, Panzetta-Dutari, Graciela M, Genti-Raimondi, Susana
Format:	Artikel
Sprache:	eng
Schlagworte:	ATP Binding Cassette Transporter, Sub-Family G, Member 2 ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Biology Biomarkers - metabolism Bromodeoxyuridine Cancer Carrier Proteins - genetics Carrier Proteins - metabolism Cell culture Cell Differentiation - genetics Cell Line, Tumor Cell migration Cell Movement - genetics Cell Proliferation Choriocarcinoma Choriocarcinoma - genetics Choriocarcinoma - pathology Chorionic Gonadotropin, beta Subunit, Human - genetics Chorionic Gonadotropin, beta Subunit, Human - metabolism Desmosomes Differentiation Experiments Gene expression Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Gene Products, env - genetics Gene Products, env - metabolism Gene Silencing Giant Cells - metabolism Humans Kinases Lipids Localization Medicine Metabolism mRNA Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Phospholipids Phospholipids - biosynthesis Pituitary (anterior) Pregnancy Proteins - genetics Pregnancy Proteins - metabolism Protein biosynthesis Protein expression Protein synthesis Proteins Reduction RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - metabolism Rodents siRNA Syncytin Thymidine Tumors Up-Regulation - genetics Wound healing
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Zusammenfassung:	StAR-related lipid transfer domain containing 7 (StarD7) is a member of the START-domain protein family whose function still remains unclear. Our data from an explorative microarray assay performed with mRNAs from StarD7 siRNA-transfected JEG-3 cells indicated that ABCG2 (ATP-binding cassette sub-family G member 2) was one of the most abundantly downregulated mRNAs. Here, we have confirmed that knocking down StarD7 mRNA lead to a decrease in the xenobiotic/lipid transporter ABCG2 at both the mRNA and protein levels (-26.4% and -41%, p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0044152