IL-15 expression on RA synovial fibroblasts promotes B cell survival

The purpose of this study was to examine the role of RA Synovial Fibroblast (RASFib) IL-15 expression on B cell survival. Magnetically sorted peripheral blood memory B cells from 15 healthy subjects were cocultured with RASFib. RASFib constitutively expressed membrane IL-15. Survival of isolated B c...

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Veröffentlicht in:	PloS one 2012-07, Vol.7 (7), p.e40620-e40620
Hauptverfasser:	Benito-Miguel, Marta, García-Carmona, Yolanda, Balsa, Alejandro, Bautista-Caro, María-Belén, Arroyo-Villa, Irene, Cobo-Ibáñez, Tatiana, Bonilla-Hernán, María Gema, Pérez de Ayala, Carlos, Sánchez-Mateos, Paloma, Martín-Mola, Emilio, Miranda-Carús, María-Eugenia
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Aged, 80 and over Apoptosis Arthritis, Rheumatoid - immunology Arthritis, Rheumatoid - metabolism B cells B-Cell Activating Factor - metabolism B-Lymphocytes - drug effects B-Lymphocytes - immunology B-Lymphocytes - metabolism Biology Blood BLyS protein Cell culture Cell survival Cell Survival - drug effects Chains Coculture Techniques Female Fibroblasts Fibroblasts - metabolism Humans Immunologic Memory Immunological memory Interleukin 15 Interleukin 15 receptors Interleukin-15 - metabolism Interleukin-15 - pharmacology Joint surgery Lymphocyte receptors Lymphocytes Lymphocytes B Male Medicine Memory cells Middle Aged Neutralizing Peripheral blood Receptors, Interleukin-15 - metabolism Survival Synovial Membrane - immunology Synovial Membrane - metabolism T cell receptors Vascular cell adhesion molecule 1 Vascular Cell Adhesion Molecule-1 - metabolism
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Zusammenfassung:	The purpose of this study was to examine the role of RA Synovial Fibroblast (RASFib) IL-15 expression on B cell survival. Magnetically sorted peripheral blood memory B cells from 15 healthy subjects were cocultured with RASFib. RASFib constitutively expressed membrane IL-15. Survival of isolated B cells cultured for 6 days, below 5%, was extended in coculture with RASFib to 52+/-8% (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0040620