T7 RNA polymerase functions in vitro without clustering

Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using 'pulldowns' and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)

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Veröffentlicht in:	PloS one 2012-07, Vol.7 (7), p.e40207-e40207
Hauptverfasser:	Finan, Kieran, Torella, Joseph P, Kapanidis, Achillefs N, Cook, Peter R
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriophage T7 - chemistry Bacteriophage T7 - metabolism Biology Chromosomes Chromosomes, Bacterial - chemistry Chromosomes, Bacterial - metabolism Clustering Clusters Coding Deoxyribonucleic acid DNA DNA polymerase DNA polymerases DNA-directed RNA polymerase DNA-Directed RNA Polymerases - chemistry DNA-Directed RNA Polymerases - metabolism E coli Elongation Enzymes Escherichia coli Escherichia coli - chemistry Escherichia coli - metabolism Fluorescence Fluorescence spectroscopy Genes Genomes Industrial plants Laboratories Microscopy Phage T7 Phages Physics Ribonucleic acid RNA RNA phages RNA polymerase RNA polymerases Spectroscopy Spectrum analysis Transcription, Genetic Viral Proteins - chemistry Viral Proteins - metabolism
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Zusammenfassung:	Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using 'pulldowns' and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0040207