Transcriptomics comparison between porcine adipose and bone marrow mesenchymal stem cells during in vitro osteogenic and adipogenic differentiation

Bone-marrow mesenchymal stem cells (BMSC) are considered the gold standard for use in tissue regeneration among mesenchymal stem cells (MSC). The abundance and ease of harvest make the adipose-derived stem cells (ASC) an attractive alternative to BMSC. The aim of the present study was to compare the...

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Veröffentlicht in:	PloS one 2012-03, Vol.7 (3), p.e32481-e32481
Hauptverfasser:	Monaco, Elisa, Bionaz, Massimo, Rodriguez-Zas, Sandra, Hurley, Walter L, Wheeler, Matthew B
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipogenesis Adipogenesis - genetics Adipose tissue Adipose Tissue - cytology Adipose Tissue - metabolism Algorithms Angiogenesis Animal sciences Animals Biocompatibility Bioinformatics Biology Biomarkers Biomedical materials Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Cell cycle Cell fate Cell growth Cell proliferation Cytoskeleton Data processing Differentiation DNA microarrays Engineering Femur Functional analysis Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Regulatory Networks Genes Genomes Genomics Guanine nucleotide-binding protein Immunity Laboratories Lipid metabolism Liver Male Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mesenchyme Metabolism Morphology Osteogenesis Osteogenesis - genetics Peroxisome proliferator-activated receptors Physiological aspects Proteins Regeneration Ribonucleic acid RNA Signal Transduction Signaling Stem cell transplantation Stem cells Studies Swine Therapeutic applications Tissue engineering Transcriptome - genetics Tumorigenesis
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Zusammenfassung:	Bone-marrow mesenchymal stem cells (BMSC) are considered the gold standard for use in tissue regeneration among mesenchymal stem cells (MSC). The abundance and ease of harvest make the adipose-derived stem cells (ASC) an attractive alternative to BMSC. The aim of the present study was to compare the transcriptome of ASC and BMSC, respectively isolated from subcutaneous adipose tissue and femur of 3 adult pigs, during in vitro osteogenic and adipogenic differentiation for up to four weeks. At 0, 2, 7, and 21 days of differentiation RNA was extracted for microarray analysis. A False Discovery Rate ≤0.05 for overall interactions effect and P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0032481