Proteomic analysis of aortae from human lipoprotein(a) transgenic mice shows an early metabolic response independent of atherosclerosis

Elevated low density lipoprotein (LDL) and lipoprotein(a) are independent risk factors for the development of atherosclerosis. Using a proteomic approach we aimed to determine early changes in arterial protein expression in transgenic mice containing both human LDL and lipoprotein(a) in circulation....

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2012-01, Vol.7 (1), p.e30383-e30383
Hauptverfasser:	Rodger, Euan J, Suetani, Rachel J, Jones, Gregory T, Kleffmann, Torsten, Carne, Alan, Legge, Michael, McCormick, Sally P A
Format:	Artikel
Sprache:	eng
Schlagworte:	Abundance Accumulation Agriculture Analysis Animals Aorta - metabolism Apolipoproteins Arteries Arteriosclerosis Atherosclerosis Atherosclerosis - blood Atherosclerosis - genetics Atherosclerosis - metabolism Biochemistry Biology Cardiovascular disease Cholesterol Clinical trials Computational Biology Coronary vessels Defense mechanisms Energy law Energy metabolism Fatty acids Female Foam Foams Gel electrophoresis Genetic engineering Genomes High density lipoprotein Humans Lipid metabolism Lipids Lipoprotein A Lipoprotein(a) - blood Lipoprotein(a) - metabolism Lipoproteins (high density) Lipoproteins (low density) Lipoproteins, LDL - blood Lipoproteins, LDL - metabolism Low density lipoprotein Low density lipoproteins Male Mass spectrometry Mass spectroscopy Metabolic response Metabolic syndrome Metabolism Mice Mice, Inbred C57BL Mice, Transgenic Oxidative stress Physiological aspects Plasma Proteins Proteomics Proteomics - methods Reverse Transcriptase Polymerase Chain Reaction Risk analysis Risk factors Rodents Smooth muscle Structural proteins Studies Toxicology Transcription Transgenic animals Transgenic mice
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Elevated low density lipoprotein (LDL) and lipoprotein(a) are independent risk factors for the development of atherosclerosis. Using a proteomic approach we aimed to determine early changes in arterial protein expression in transgenic mice containing both human LDL and lipoprotein(a) in circulation. Plasma lipid analyses showed the lipoprotein(a) transgenic mice had significantly higher lipid levels than wildtype, including a much increased LDL and high density lipoprotein (HDL) cholesterol. Analysis of aortae from lipoprotein(a) mice showed lipoprotein(a) accumulation but no lipid accumulation or foam cells, leaving the arteries essentially atherosclerosis free. Using two-dimensional gel electrophoresis and mass spectrometry, we identified 34 arterial proteins with significantly altered abundance (P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0030383