Pre-treatment of recombinant mouse MFG-E8 downregulates LPS-induced TNF-α production in macrophages via STAT3-mediated SOCS3 activation

Milk fat globule-epidermal growth factor factor 8 (MFG-E8) regulates innate immune function by modulating cellular signaling, which is less understood. Herein, we aimed to investigate the direct anti-inflammatory role of MFG-E8 in macrophages by pre-treatment with recombinant murine MFG-E8 (rmMFG-E8...

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Veröffentlicht in:	PloS one 2011, Vol.6 (11), p.e27685-e27685
Hauptverfasser:	Aziz, Monowar, Jacob, Asha, Matsuda, Akihisa, Wu, Rongqian, Zhou, Mian, Dong, Weifeng, Yang, Weng-Lang, Wang, Ping
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal tissues Animals Antigens, Surface - physiology Apoptosis Binding sites Biology Blotting, Western Breast cancer Cell activation Cells, Cultured Cytokines Disease Models, Animal Drosophila Endotoxemia Endotoxemia - drug therapy Endotoxemia - metabolism Enzyme-Linked Immunosorbent Assay Enzymes Epidermal growth factor Immune response Immune system Immunology Immunoprecipitation Inflammation Inflammatory diseases Insects Intensive care Kinases Ligands Lipopolysaccharides Lipopolysaccharides - pharmacology Macrophages Macrophages, Peritoneal - cytology Macrophages, Peritoneal - metabolism Male Medical research Medicine Mice Mice, Inbred C57BL Mice, Knockout Milk Milk fat globule membranes Milk Proteins mRNA NF-κB protein Peritoneum Phagocytosis Pretreatment Proteins Real-Time Polymerase Chain Reaction Recombinant Proteins - genetics Recombinant Proteins - metabolism RNA, Messenger - genetics Rodents Sepsis Signal Transduction Signaling Spleen Stat3 protein STAT3 Transcription Factor - genetics STAT3 Transcription Factor - metabolism Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins - genetics Suppressor of Cytokine Signaling Proteins - metabolism Surgery TLR4 protein Toll-like receptors Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-TNF Tumor necrosis factor-α
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Zusammenfassung:	Milk fat globule-epidermal growth factor factor 8 (MFG-E8) regulates innate immune function by modulating cellular signaling, which is less understood. Herein, we aimed to investigate the direct anti-inflammatory role of MFG-E8 in macrophages by pre-treatment with recombinant murine MFG-E8 (rmMFG-E8) followed by stimulation with LPS in RAW264.7 cells and in peritoneal macrophages, isolated from wild-type (WT) or MFG-E8(-/-) mice. RAW264.7 cells and mouse peritoneal macrophages treated with rmMFG-E8 significantly downregulated LPS-induced TNF-α mRNA by 25% and 24%, and protein levels by 29% and 23%, respectively (P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0027685