Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs
We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We...
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| description | We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We observed that IL-12 and IFNγ production from mitogenic stimulated peripheral blood mononuclear cells decreased (P |
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Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We observed that IL-12 and IFNγ production from mitogenic stimulated peripheral blood mononuclear cells decreased (P<0.10) following 31 days of GM maize exposure. While Cry1Ab-specific IgG and IgA were not detected in the plasma of GM maize-fed pigs, the detection of the cry1Ab gene and protein was limited to the gastrointestinal digesta and was not found in the kidneys, liver, spleen, muscle, heart or blood. Feeding GM maize to weanling pigs had no effect on growth performance or body weight. IL-6 and IL-4 production from isolated splenocytes were increased (P<0.05) in response to feeding GM maize while the proportion of CD4(+) T cells in the spleen decreased. In the ileum, the proportion of B cells and macrophages decreased while the proportion of CD4(+) T cells increased in GM maize-fed pigs. IL-8 and IL-4 production from isolated intraepithelial and lamina propria lymphocytes were also increased (P<0.05) in response to feeding GM maize. In conclusion, there was no evidence of cry1Ab gene or protein translocation to the organs and blood of weaning pigs. The growth of pigs was not affected by feeding GM maize. Alterations in immune responses were detected; however, their biologic relevance is questionable.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0027177</identifier><identifier>PMID: 22132091</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acclimatization ; Administration, Oral ; Agricultural production ; Agriculture ; Animals ; B cells ; Bacillus thuringiensis ; Bacillus thuringiensis - physiology ; Bacterial Proteins - blood ; Bacterial Proteins - metabolism ; Biology ; Blood ; Body Weight ; CD4 antigen ; Corn ; Crops ; Cry1ab gene ; Deoxyribonucleic acid ; Diet ; DNA ; DNA, Plant - administration & dosage ; DNA, Plant - genetics ; Endotoxins - blood ; Endotoxins - metabolism ; Experiments ; Farmers ; Feeding ; Feeding Behavior ; Feeds ; Food ; Food, Genetically Modified ; Genes, Plant - genetics ; Genetic engineering ; Genetic modification ; Genetically altered foods ; Genetically engineered foods ; Genetically modified crops ; Grasslands ; Growth ; Heart ; Hemolysin Proteins - blood ; Hemolysin Proteins - metabolism ; Hogs ; Ileum ; Immune response ; Immune system ; Immunity - immunology ; Immunoglobulin A ; Immunoglobulin G ; Interleukin 12 ; Interleukin 4 ; Interleukin 6 ; Interleukin 8 ; Kidneys ; Lamina propria ; Leukocytes (mononuclear) ; Leukocytes - metabolism ; Life sciences ; Liver ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Macrophages ; Mycotoxins - analysis ; Nutrition ; Oral administration ; Organ Specificity ; Organs ; Peripheral blood mononuclear cells ; Pesticide Residues - analysis ; Plants, Genetically Modified ; Polymerase Chain Reaction ; Protein transport ; Proteins ; Soybeans ; Spleen ; Studies ; Swine ; Swine - growth & development ; Swine - immunology ; T cells ; Time Factors ; Transgenes - genetics ; Transgenic plants ; Translocation ; Weaning ; Zea mays - genetics ; Zea mays - microbiology ; γ-Interferon</subject><ispartof>PloS one, 2011-11, Vol.6 (11), p.e27177</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Walsh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Walsh et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-169f5cfdb389a4f0f13e147450d52109babc8e6bc8b9df911ec609909a58921f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223173/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223173/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22132091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Walsh, Maria C</creatorcontrib><creatorcontrib>Buzoianu, Stefan G</creatorcontrib><creatorcontrib>Gardiner, Gillian E</creatorcontrib><creatorcontrib>Rea, Mary C</creatorcontrib><creatorcontrib>Gelencsér, Eva</creatorcontrib><creatorcontrib>Jánosi, Anna</creatorcontrib><creatorcontrib>Epstein, Michelle M</creatorcontrib><creatorcontrib>Ross, R Paul</creatorcontrib><creatorcontrib>Lawlor, Peadar G</creatorcontrib><title>Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We observed that IL-12 and IFNγ production from mitogenic stimulated peripheral blood mononuclear cells decreased (P<0.10) following 31 days of GM maize exposure. While Cry1Ab-specific IgG and IgA were not detected in the plasma of GM maize-fed pigs, the detection of the cry1Ab gene and protein was limited to the gastrointestinal digesta and was not found in the kidneys, liver, spleen, muscle, heart or blood. Feeding GM maize to weanling pigs had no effect on growth performance or body weight. IL-6 and IL-4 production from isolated splenocytes were increased (P<0.05) in response to feeding GM maize while the proportion of CD4(+) T cells in the spleen decreased. In the ileum, the proportion of B cells and macrophages decreased while the proportion of CD4(+) T cells increased in GM maize-fed pigs. IL-8 and IL-4 production from isolated intraepithelial and lamina propria lymphocytes were also increased (P<0.05) in response to feeding GM maize. In conclusion, there was no evidence of cry1Ab gene or protein translocation to the organs and blood of weaning pigs. The growth of pigs was not affected by feeding GM maize. Alterations in immune responses were detected; however, their biologic relevance is questionable.</description><subject>Acclimatization</subject><subject>Administration, Oral</subject><subject>Agricultural production</subject><subject>Agriculture</subject><subject>Animals</subject><subject>B cells</subject><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis - physiology</subject><subject>Bacterial Proteins - blood</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biology</subject><subject>Blood</subject><subject>Body Weight</subject><subject>CD4 antigen</subject><subject>Corn</subject><subject>Crops</subject><subject>Cry1ab gene</subject><subject>Deoxyribonucleic acid</subject><subject>Diet</subject><subject>DNA</subject><subject>DNA, Plant - administration & dosage</subject><subject>DNA, Plant - genetics</subject><subject>Endotoxins - blood</subject><subject>Endotoxins - metabolism</subject><subject>Experiments</subject><subject>Farmers</subject><subject>Feeding</subject><subject>Feeding Behavior</subject><subject>Feeds</subject><subject>Food</subject><subject>Food, Genetically Modified</subject><subject>Genes, Plant - genetics</subject><subject>Genetic engineering</subject><subject>Genetic modification</subject><subject>Genetically altered foods</subject><subject>Genetically engineered foods</subject><subject>Genetically modified crops</subject><subject>Grasslands</subject><subject>Growth</subject><subject>Heart</subject><subject>Hemolysin Proteins - blood</subject><subject>Hemolysin Proteins - metabolism</subject><subject>Hogs</subject><subject>Ileum</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunity - immunology</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin G</subject><subject>Interleukin 12</subject><subject>Interleukin 4</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Kidneys</subject><subject>Lamina propria</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes - metabolism</subject><subject>Life sciences</subject><subject>Liver</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Mycotoxins - analysis</subject><subject>Nutrition</subject><subject>Oral administration</subject><subject>Organ Specificity</subject><subject>Organs</subject><subject>Peripheral blood mononuclear cells</subject><subject>Pesticide Residues - analysis</subject><subject>Plants, Genetically Modified</subject><subject>Polymerase Chain Reaction</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Soybeans</subject><subject>Spleen</subject><subject>Studies</subject><subject>Swine</subject><subject>Swine - growth & development</subject><subject>Swine - immunology</subject><subject>T cells</subject><subject>Time Factors</subject><subject>Transgenes - genetics</subject><subject>Transgenic plants</subject><subject>Translocation</subject><subject>Weaning</subject><subject>Zea mays - genetics</subject><subject>Zea mays - microbiology</subject><subject>γ-Interferon</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl9rFDEUxQdRbK1-A9GAIPiwa24yk5m8CGu1ulC74L_XkMkks1lmJtsko9ZPb9qdll1QkIQkJL97cjmcLHsKeA60hNcbN_pBdvOtG_QcY1JCWd7LjoFTMmME0_t756PsUQgbjAtaMfYwOyIEKMEcjrPLMxk1cgZFL4fQ6sEq9O5igYx3PXJedt0Vkk1vBxui9rpBbyP6tLqoAKNe2t8ayaFB2hitYkBuQLbvx0Ejr0PqK-yeW-9-xjWyA9raNjzOHhjZBf1k2k-yb2fvv55-nJ2vPixPF-czxTjEGTBuCmWamlZc5gYboBryMi9wUxDAvJa1qjRLS80bwwG0YphzzGVRcQKGnmTPd7rbzgUxuRUEUMCEcFaQRCx3ROPkRmy97aW_Ek5acXPhfCukj1Z1WsiyqBmATFPlTJuaqBxqig03wGSFk9ab6bex7nWj9JAM7Q5ED18Guxat-yEoIRRKmgReTALeXY46xH-0PFGtTF3ZwbgkpnoblFjkJasYVDda879QaTS6tyrlxdh0f1Dw6qAgMVH_iq0cQxDLL5__n119P2Rf7rFrLbu4Dq4bo03hOATzHai8C8Frc-ccYHEd91s3xHXcxRT3VPZs3_W7ott80z-lIvna</recordid><startdate>20111123</startdate><enddate>20111123</enddate><creator>Walsh, Maria C</creator><creator>Buzoianu, Stefan G</creator><creator>Gardiner, Gillian E</creator><creator>Rea, Mary C</creator><creator>Gelencsér, Eva</creator><creator>Jánosi, Anna</creator><creator>Epstein, Michelle M</creator><creator>Ross, R Paul</creator><creator>Lawlor, Peadar G</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20111123</creationdate><title>Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs</title><author>Walsh, Maria C ; Buzoianu, Stefan G ; Gardiner, Gillian E ; Rea, Mary C ; Gelencsér, Eva ; Jánosi, Anna ; Epstein, Michelle M ; Ross, R Paul ; Lawlor, Peadar G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-169f5cfdb389a4f0f13e147450d52109babc8e6bc8b9df911ec609909a58921f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acclimatization</topic><topic>Administration, Oral</topic><topic>Agricultural production</topic><topic>Agriculture</topic><topic>Animals</topic><topic>B cells</topic><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis - physiology</topic><topic>Bacterial Proteins - blood</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biology</topic><topic>Blood</topic><topic>Body Weight</topic><topic>CD4 antigen</topic><topic>Corn</topic><topic>Crops</topic><topic>Cry1ab gene</topic><topic>Deoxyribonucleic acid</topic><topic>Diet</topic><topic>DNA</topic><topic>DNA, Plant - administration & dosage</topic><topic>DNA, Plant - genetics</topic><topic>Endotoxins - blood</topic><topic>Endotoxins - metabolism</topic><topic>Experiments</topic><topic>Farmers</topic><topic>Feeding</topic><topic>Feeding Behavior</topic><topic>Feeds</topic><topic>Food</topic><topic>Food, Genetically Modified</topic><topic>Genes, Plant - genetics</topic><topic>Genetic engineering</topic><topic>Genetic modification</topic><topic>Genetically altered foods</topic><topic>Genetically engineered foods</topic><topic>Genetically modified crops</topic><topic>Grasslands</topic><topic>Growth</topic><topic>Heart</topic><topic>Hemolysin Proteins - 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Pigs were fed a diet containing 38.9% GM or non-GM isogenic parent line maize for 31 days. We observed that IL-12 and IFNγ production from mitogenic stimulated peripheral blood mononuclear cells decreased (P<0.10) following 31 days of GM maize exposure. While Cry1Ab-specific IgG and IgA were not detected in the plasma of GM maize-fed pigs, the detection of the cry1Ab gene and protein was limited to the gastrointestinal digesta and was not found in the kidneys, liver, spleen, muscle, heart or blood. Feeding GM maize to weanling pigs had no effect on growth performance or body weight. IL-6 and IL-4 production from isolated splenocytes were increased (P<0.05) in response to feeding GM maize while the proportion of CD4(+) T cells in the spleen decreased. In the ileum, the proportion of B cells and macrophages decreased while the proportion of CD4(+) T cells increased in GM maize-fed pigs. IL-8 and IL-4 production from isolated intraepithelial and lamina propria lymphocytes were also increased (P<0.05) in response to feeding GM maize. In conclusion, there was no evidence of cry1Ab gene or protein translocation to the organs and blood of weaning pigs. The growth of pigs was not affected by feeding GM maize. Alterations in immune responses were detected; however, their biologic relevance is questionable.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22132091</pmid><doi>10.1371/journal.pone.0027177</doi><tpages>e27177</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2011-11, Vol.6 (11), p.e27177 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1310229652 |
| source | PubMed (Medline); PLoS; MEDLINE; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Acclimatization Administration, Oral Agricultural production Agriculture Animals B cells Bacillus thuringiensis Bacillus thuringiensis - physiology Bacterial Proteins - blood Bacterial Proteins - metabolism Biology Blood Body Weight CD4 antigen Corn Crops Cry1ab gene Deoxyribonucleic acid Diet DNA DNA, Plant - administration & dosage DNA, Plant - genetics Endotoxins - blood Endotoxins - metabolism Experiments Farmers Feeding Feeding Behavior Feeds Food Food, Genetically Modified Genes, Plant - genetics Genetic engineering Genetic modification Genetically altered foods Genetically engineered foods Genetically modified crops Grasslands Growth Heart Hemolysin Proteins - blood Hemolysin Proteins - metabolism Hogs Ileum Immune response Immune system Immunity - immunology Immunoglobulin A Immunoglobulin G Interleukin 12 Interleukin 4 Interleukin 6 Interleukin 8 Kidneys Lamina propria Leukocytes (mononuclear) Leukocytes - metabolism Life sciences Liver Lymphocytes Lymphocytes B Lymphocytes T Macrophages Mycotoxins - analysis Nutrition Oral administration Organ Specificity Organs Peripheral blood mononuclear cells Pesticide Residues - analysis Plants, Genetically Modified Polymerase Chain Reaction Protein transport Proteins Soybeans Spleen Studies Swine Swine - growth & development Swine - immunology T cells Time Factors Transgenes - genetics Transgenic plants Translocation Weaning Zea mays - genetics Zea mays - microbiology γ-Interferon |
| title | Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs |
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