Quantitative analysis and diagnostic significance of methylated SLC19A3 DNA in the plasma of breast and gastric cancer patients

Previously, we have examined the methylation status of SLC19A3 (solute carrier family 19, member 3) promoter and found that SLC19A3 was epigenetically down-regulated in gastric cancer. Here, we aim to develop a new biomarker for cancer diagnosis using methylated SLC19A3 DNA in plasma. SLC19A3 gene e...

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Veröffentlicht in:	PloS one 2011-07, Vol.6 (7), p.e22233-e22233
Hauptverfasser:	Ng, Enders K O, Leung, Candy P H, Shin, Vivian Y, Wong, Chris L P, Ma, Edmond S K, Jin, Hong Chuan, Chu, Kent-Man, Kwong, Ava
Format:	Artikel
Sprache:	eng
Schlagworte:	Aberration Adult Aged Aged, 80 and over Analysis Apoptosis Biology Biomarkers Breast cancer Breast Neoplasms - blood Breast Neoplasms - diagnosis Breast Neoplasms - genetics Breast Neoplasms - pathology Cancer Cancer diagnosis Cancer genetics Cancer patients Care and treatment Colorectal cancer Deoxyribonucleic acid Diagnosis Diagnostic systems DNA DNA methylation DNA Methylation - genetics Down-Regulation - genetics Enzymes Epigenetics Female Gastric cancer Gene expression Gene Expression Regulation, Neoplastic Genes Hospitals Humans Male Medical diagnosis Medical screening Medicine Membrane Transport Proteins - genetics Metabolism Methylation MicroRNAs Middle Aged Mitochondrial DNA Neoplasm Staging Pathology Patients Plasma Promoter Regions, Genetic - genetics Quantitative analysis Reproducibility of Results Review boards ROC Curve Stomach cancer Stomach Neoplasms - blood Stomach Neoplasms - diagnosis Stomach Neoplasms - genetics Stomach Neoplasms - pathology Surgery Tissues Tumors Vitamin B
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Zusammenfassung:	Previously, we have examined the methylation status of SLC19A3 (solute carrier family 19, member 3) promoter and found that SLC19A3 was epigenetically down-regulated in gastric cancer. Here, we aim to develop a new biomarker for cancer diagnosis using methylated SLC19A3 DNA in plasma. SLC19A3 gene expression was examined by RT-qPCR. Methylation status of SLC19A3 promoter was evaluated by methylation-specific qPCR. SLC19A3 expression was significantly down-regulated in 80% (12/15) of breast tumors (P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0022233