Surveillance of transmitted antiretroviral drug resistance among HIV-1 infected women attending antenatal clinics in Chitungwiza, Zimbabwe
The rapid scale-up of highly active antiretroviral therapy (HAART) and use of single dose Nevirapine (SD NVP) for prevention of mother-to-child transmission (pMTCT) have raised fears about the emergence of resistance to the first line antiretroviral drug regimens. A cross-sectional study was conduct...
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| Veröffentlicht in: | PloS one 2011-06, Vol.6 (6), p.e21241-e21241 |
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| creator | Tshabalala, Mqondisi Manasa, Justen Zijenah, Lynn S Rusakaniko, Simbarashe Kadzirange, Gerard Mucheche, Mary Kassaye, Seble Johnston, Elizabeth Katzenstein, David |
| description | The rapid scale-up of highly active antiretroviral therapy (HAART) and use of single dose Nevirapine (SD NVP) for prevention of mother-to-child transmission (pMTCT) have raised fears about the emergence of resistance to the first line antiretroviral drug regimens. A cross-sectional study was conducted to determine the prevalence of primary drug resistance (PDR) in a cohort of young ( |
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A cross-sectional study was conducted to determine the prevalence of primary drug resistance (PDR) in a cohort of young (<25 yrs) HAART-naïve HIV pregnant women attending antenatal clinics in Chitungwiza, Zimbabwe. Whole blood was collected in EDTA for CD4 counts, viral load, serological estimation of duration of infection using the BED Calypte assay and genotyping for drug resistance. Four hundred and seventy-one women, mean age 21 years; SD: 2.1 were enrolled into the study between 2006 and 2007. Their median CD4 count was 371cells/µL; IQR: 255-511 cells/µL. Two hundred and thirty-six samples were genotyped for drug resistance. Based on the BED assay, 27% were recently infected (RI) whilst 73% had long-term infection (LTI). Median CD4 count was higher (p<0.05) in RI than in women with LTI. Only 2 women had drug resistance mutations; protease I85V and reverse transcriptase Y181C. Prevalence of PDR in Chitungwiza, 4 years after commencement of the national ART program remained below WHO threshold limit (5%). Frequency of recent infection BED testing is consistent with high HIV acquisition during pregnancy. With the scale-up of long-term ART programs, maintenance of proper prescribing practices, continuous monitoring of patients and reinforcement of adherence may prevent the acquisition and transmission of PDR.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0021241</identifier><identifier>PMID: 21698125</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acquired immune deficiency syndrome ; Adult ; AIDS ; Antiretroviral agents ; Antiretroviral drugs ; Bed load ; Biology ; CD4 antigen ; CD4 Lymphocyte Count ; DNA polymerases ; Drug resistance ; Drug Resistance, Viral ; Drug therapy ; Ethylenediaminetetraacetic acids ; Female ; Genotyping ; Highly active antiretroviral therapy ; HIV ; HIV Infections - drug therapy ; HIV Infections - epidemiology ; HIV Infections - virology ; HIV-1 - isolation & purification ; Human immunodeficiency virus ; Humans ; Infection ; Infections ; Medicine ; Mutation ; Nevirapine ; Population Surveillance ; Pregnancy ; Pregnant women ; Proteases ; RNA-directed DNA polymerase ; Viral Load ; Womens health ; Zimbabwe - epidemiology</subject><ispartof>PloS one, 2011-06, Vol.6 (6), p.e21241-e21241</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c757t-69e0f41014d3504221033eba0f04ffc35ed4b5558d90cb496bc7cbdde681ac643</citedby><cites>FETCH-LOGICAL-c757t-69e0f41014d3504221033eba0f04ffc35ed4b5558d90cb496bc7cbdde681ac643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116901/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116901/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21698125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Stoddart, Cheryl A.</contributor><creatorcontrib>Tshabalala, Mqondisi</creatorcontrib><creatorcontrib>Manasa, Justen</creatorcontrib><creatorcontrib>Zijenah, Lynn S</creatorcontrib><creatorcontrib>Rusakaniko, Simbarashe</creatorcontrib><creatorcontrib>Kadzirange, Gerard</creatorcontrib><creatorcontrib>Mucheche, Mary</creatorcontrib><creatorcontrib>Kassaye, Seble</creatorcontrib><creatorcontrib>Johnston, Elizabeth</creatorcontrib><creatorcontrib>Katzenstein, David</creatorcontrib><title>Surveillance of transmitted antiretroviral drug resistance among HIV-1 infected women attending antenatal clinics in Chitungwiza, Zimbabwe</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The rapid scale-up of highly active antiretroviral therapy (HAART) and use of single dose Nevirapine (SD NVP) for prevention of mother-to-child transmission (pMTCT) have raised fears about the emergence of resistance to the first line antiretroviral drug regimens. A cross-sectional study was conducted to determine the prevalence of primary drug resistance (PDR) in a cohort of young (<25 yrs) HAART-naïve HIV pregnant women attending antenatal clinics in Chitungwiza, Zimbabwe. Whole blood was collected in EDTA for CD4 counts, viral load, serological estimation of duration of infection using the BED Calypte assay and genotyping for drug resistance. Four hundred and seventy-one women, mean age 21 years; SD: 2.1 were enrolled into the study between 2006 and 2007. Their median CD4 count was 371cells/µL; IQR: 255-511 cells/µL. Two hundred and thirty-six samples were genotyped for drug resistance. Based on the BED assay, 27% were recently infected (RI) whilst 73% had long-term infection (LTI). Median CD4 count was higher (p<0.05) in RI than in women with LTI. Only 2 women had drug resistance mutations; protease I85V and reverse transcriptase Y181C. Prevalence of PDR in Chitungwiza, 4 years after commencement of the national ART program remained below WHO threshold limit (5%). Frequency of recent infection BED testing is consistent with high HIV acquisition during pregnancy. With the scale-up of long-term ART programs, maintenance of proper prescribing practices, continuous monitoring of patients and reinforcement of adherence may prevent the acquisition and transmission of PDR.</description><subject>Acquired immune deficiency syndrome</subject><subject>Adult</subject><subject>AIDS</subject><subject>Antiretroviral agents</subject><subject>Antiretroviral drugs</subject><subject>Bed load</subject><subject>Biology</subject><subject>CD4 antigen</subject><subject>CD4 Lymphocyte Count</subject><subject>DNA polymerases</subject><subject>Drug resistance</subject><subject>Drug Resistance, Viral</subject><subject>Drug therapy</subject><subject>Ethylenediaminetetraacetic acids</subject><subject>Female</subject><subject>Genotyping</subject><subject>Highly active antiretroviral therapy</subject><subject>HIV</subject><subject>HIV Infections - drug therapy</subject><subject>HIV Infections - epidemiology</subject><subject>HIV Infections - virology</subject><subject>HIV-1 - isolation & purification</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infection</subject><subject>Infections</subject><subject>Medicine</subject><subject>Mutation</subject><subject>Nevirapine</subject><subject>Population Surveillance</subject><subject>Pregnancy</subject><subject>Pregnant women</subject><subject>Proteases</subject><subject>RNA-directed DNA polymerase</subject><subject>Viral Load</subject><subject>Womens health</subject><subject>Zimbabwe - epidemiology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9GK1DAUhoso7rr6BqIFQRGcMWnStL0RlkHdgYUFV_fCm5Amp50MbTIm6az6CD616U53mZG9kF60JN__n-Q_PUnyHKM5JgV-v7aDM6Kbb6yBOUIZzih-kBzjimQzliHycO_7KHni_RqhnJSMPU6OMsyqEmf5cfLncnBb0F0njITUNmlwwvhehwAqFSZoB8HZrXaiS5Ub2tSB1z7c0KK3pk3PllcznGrTgBw117YHk4qoN0rH7egBRoQol502WvqIpouVDoNpr_Vv8S79rvta1NfwNHnUiM7Ds-l9knz79PHr4mx2fvF5uTg9n8kiL8KMVYAaihGmiuSIZhlGhEAtUINo00iSg6J1nuelqpCsacVqWchaKWAlFpJRcpK83PluOuv5FKPnmCDKSM6qLBLLHaGsWPON071wv7gVmt8sWNdy4YKWHfAyx4KVTYbrmH8moM4JQhVQqnIgBI_VPkzVhroHJcHEhLsD08Mdo1e8tVtOcGwSwtHgzWTg7I8BfOC99hLGjoEdPC8LUiBKCxTJV_-Q919uoloRzx_7ZmNZOXryU1qwsiwqzCI1v4eKj4Jey_jLNTquHwjeHggiE-BnaMXgPV9efvl_9uLqkH29x65AdGHlbTcEbY0_BOkOlM5676C5yxgjPk7MbRp8nBg-TUyUvdjvz53odkTIX4-XEqw</recordid><startdate>20110616</startdate><enddate>20110616</enddate><creator>Tshabalala, Mqondisi</creator><creator>Manasa, Justen</creator><creator>Zijenah, Lynn S</creator><creator>Rusakaniko, Simbarashe</creator><creator>Kadzirange, Gerard</creator><creator>Mucheche, Mary</creator><creator>Kassaye, Seble</creator><creator>Johnston, Elizabeth</creator><creator>Katzenstein, David</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110616</creationdate><title>Surveillance of transmitted antiretroviral drug resistance among HIV-1 infected women attending antenatal clinics in Chitungwiza, Zimbabwe</title><author>Tshabalala, Mqondisi ; Manasa, Justen ; Zijenah, Lynn S ; Rusakaniko, Simbarashe ; Kadzirange, Gerard ; Mucheche, Mary ; Kassaye, Seble ; Johnston, Elizabeth ; Katzenstein, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c757t-69e0f41014d3504221033eba0f04ffc35ed4b5558d90cb496bc7cbdde681ac643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acquired immune deficiency syndrome</topic><topic>Adult</topic><topic>AIDS</topic><topic>Antiretroviral agents</topic><topic>Antiretroviral drugs</topic><topic>Bed load</topic><topic>Biology</topic><topic>CD4 antigen</topic><topic>CD4 Lymphocyte Count</topic><topic>DNA polymerases</topic><topic>Drug resistance</topic><topic>Drug Resistance, Viral</topic><topic>Drug therapy</topic><topic>Ethylenediaminetetraacetic acids</topic><topic>Female</topic><topic>Genotyping</topic><topic>Highly active antiretroviral therapy</topic><topic>HIV</topic><topic>HIV Infections - 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A cross-sectional study was conducted to determine the prevalence of primary drug resistance (PDR) in a cohort of young (<25 yrs) HAART-naïve HIV pregnant women attending antenatal clinics in Chitungwiza, Zimbabwe. Whole blood was collected in EDTA for CD4 counts, viral load, serological estimation of duration of infection using the BED Calypte assay and genotyping for drug resistance. Four hundred and seventy-one women, mean age 21 years; SD: 2.1 were enrolled into the study between 2006 and 2007. Their median CD4 count was 371cells/µL; IQR: 255-511 cells/µL. Two hundred and thirty-six samples were genotyped for drug resistance. Based on the BED assay, 27% were recently infected (RI) whilst 73% had long-term infection (LTI). Median CD4 count was higher (p<0.05) in RI than in women with LTI. Only 2 women had drug resistance mutations; protease I85V and reverse transcriptase Y181C. Prevalence of PDR in Chitungwiza, 4 years after commencement of the national ART program remained below WHO threshold limit (5%). Frequency of recent infection BED testing is consistent with high HIV acquisition during pregnancy. With the scale-up of long-term ART programs, maintenance of proper prescribing practices, continuous monitoring of patients and reinforcement of adherence may prevent the acquisition and transmission of PDR.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21698125</pmid><doi>10.1371/journal.pone.0021241</doi><tpages>e21241</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2011-06, Vol.6 (6), p.e21241-e21241 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Acquired immune deficiency syndrome Adult AIDS Antiretroviral agents Antiretroviral drugs Bed load Biology CD4 antigen CD4 Lymphocyte Count DNA polymerases Drug resistance Drug Resistance, Viral Drug therapy Ethylenediaminetetraacetic acids Female Genotyping Highly active antiretroviral therapy HIV HIV Infections - drug therapy HIV Infections - epidemiology HIV Infections - virology HIV-1 - isolation & purification Human immunodeficiency virus Humans Infection Infections Medicine Mutation Nevirapine Population Surveillance Pregnancy Pregnant women Proteases RNA-directed DNA polymerase Viral Load Womens health Zimbabwe - epidemiology |
| title | Surveillance of transmitted antiretroviral drug resistance among HIV-1 infected women attending antenatal clinics in Chitungwiza, Zimbabwe |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T03%3A34%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Surveillance%20of%20transmitted%20antiretroviral%20drug%20resistance%20among%20HIV-1%20infected%20women%20attending%20antenatal%20clinics%20in%20Chitungwiza,%20Zimbabwe&rft.jtitle=PloS%20one&rft.au=Tshabalala,%20Mqondisi&rft.date=2011-06-16&rft.volume=6&rft.issue=6&rft.spage=e21241&rft.epage=e21241&rft.pages=e21241-e21241&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0021241&rft_dat=%3Cgale_plos_%3EA476887916%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1304635692&rft_id=info:pmid/21698125&rft_galeid=A476887916&rft_doaj_id=oai_doaj_org_article_851a68f21b1242aeb53009e44d5e3314&rfr_iscdi=true |