Profiling the dead : generating microsatellite data from fossil bones of extinct megafauna - protocols, problems, and prospects

Presents the first set of microsatellite markers developed exclusively for an extinct taxon with low copy number (LCN) DNA - the extinct New Zealand moa (Aves: Dinornithiformes). Demonstrates the viability of the primers and the protocols used, by compiling a full Dinornis microsatellite dataset rep...

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Veröffentlicht in:	PloS one 2011-01, Vol.6 (1), p.e16670-e16670
1. Verfasser:	Allentoft, Morten E
Format:	Artikel
Sprache:	eng
Schlagworte:	Agkistrodon contortrix Analysis Animals Animals, Fossil Aves Biology Biotechnology Birds Birds, Fossil Bone and Bones Bones Copy number Data processing Deoxyribonucleic acid Dinornis DNA DNA Primers DNA sequencing DNA, Fossil DNA, Mitochondrial - isolation & purification Egg Shell Egg shells Endangered & extinct species Extinct birds Extinction Extinction, Biological Fossils Gene sequencing Genetic aspects Genetic diversity Genetic markers Genetics Genomes Identification Laboratories Leiopelma hochstetteri Loci Markers Megafauna Microsatellite Repeats - genetics Microsatellites Microsatellites (Genetics) Mitochondrial DNA Moa Museums Natural history Next-generation sequencing Palaeognathae Polymerase chain reaction Polymorphism Population genetics Preservation Primers Research Design Single nucleotide polymorphisms Single-nucleotide polymorphism Studies Substrates Technology Viability Wildlife conservation
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Beschreibung
Zusammenfassung:	Presents the first set of microsatellite markers developed exclusively for an extinct taxon with low copy number (LCN) DNA - the extinct New Zealand moa (Aves: Dinornithiformes). Demonstrates the viability of the primers and the protocols used, by compiling a full Dinornis microsatellite dataset representing fossils of c. 600–5000 years of age. Source: National Library of New Zealand Te Puna Matauranga o Aotearoa, licensed by the Department of Internal Affairs for re-use under the Creative Commons Attribution 3.0 New Zealand Licence.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0016670