A systematic proteomic study of irradiated DNA repair deficient Nbn-mice

The NBN gene codes for the protein nibrin, which is involved in the detection and repair of DNA double strand breaks (DSBs). The NBN gene is essential in mammals. We have used a conditional null mutant mouse model in a proteomics approach to identify proteins with modified expression levels after 4...

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Veröffentlicht in:	PloS one 2009-05, Vol.4 (5), p.e5423-e5423
Hauptverfasser:	Melchers, Anna, Stöckl, Lars, Radszewski, Janina, Anders, Marco, Krenzlin, Harald, Kalischke, Candy, Scholz, Regina, Jordan, Andreas, Nebrich, Grit, Klose, Joachim, Sperling, Karl, Digweed, Martin, Demuth, Ilja
Format:	Artikel
Sprache:	eng
Schlagworte:	Analgesics Animals Ataxia Autosomal recessive inheritance Biochemistry Breakage Cancer Cell Biology/Chemical Biology of the Cell Cell Biology/Gene Expression Cell cycle Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Deoxyribonucleic acid DNA DNA Breaks, Double-Stranded - radiation effects DNA damage DNA repair DNA Repair - genetics DNA Repair - physiology Efficiency Electrophoresis, Gel, Two-Dimensional Fibroblasts Gender differences Gene Expression Genetics and Genomics/Cancer Genetics Genetics and Genomics/Disease Models Genetics and Genomics/Medical Genetics Growth rate Health risks Heat-Shock Response Homeostasis Interferon Irradiation Kinases Liver - metabolism Liver - radiation effects Malignancy Mammals Mice Mice, Knockout Molecular Chaperones - metabolism Mutants Mutation NAD - metabolism Nanotechnology Nijmegen breakage syndrome Nuclear Proteins - deficiency Nuclear Proteins - genetics Nuclear Proteins - metabolism Oxidation-Reduction Oxidative Stress Oxygen Proteins Proteome - isolation & purification Proteome - metabolism Proteomics Radiosensitivity Reactive oxygen species Reactive Oxygen Species - metabolism Repair Rodents
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Zusammenfassung:	The NBN gene codes for the protein nibrin, which is involved in the detection and repair of DNA double strand breaks (DSBs). The NBN gene is essential in mammals. We have used a conditional null mutant mouse model in a proteomics approach to identify proteins with modified expression levels after 4 Gy ionizing irradiation in the absence of nibrin in vivo. Altogether, amongst approximately 8,000 resolved proteins, 209 were differentially expressed in homozygous null mutant mice in comparison to control animals. One group of proteins significantly altered in null mutant mice were those involved in oxidative stress and cellular redox homeostasis (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0005423