CD8+ T Cells as a Source of IFN-[gamma] Production in Human Cutaneous Leishmaniasis: e845

CD8+ T Cells as a Source of IFN-[gamma] Production in Human Cutaneous Leishmaniasis: e845

Background In human leishmaniasis Th1/Th2 dichotomy similar to murine model is not clearly defined and surrogate marker(s) of protection is not yet known. In this study, Th1/Th2 cytokines (IL-5, IL-10, IL-13 and IFN-γ) profile induced by purified CD4+/CD8+ T cells in response to Leishmania antigens...

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Veröffentlicht in:PLoS neglected tropical diseases 2010-10, Vol.4 (10)
Hauptverfasser: Rostami, Mahmoud Nateghi, Keshavarz, Hossein, Edalat, Rosita, Sarrafnejad, Abdolfattah, Shahrestani, Tahereh, Mahboudi, Fereidoun, Khamesipour, Ali
Format: Artikel
Sprache:eng
Schlagworte:
Antigens
Cytokines
Gene expression
HIV
Human immunodeficiency virus
Immune response
Infections
Lymphocytes
Parasites
Parasitic diseases
Protozoa
Tropical diseases
Vaccines
Vector-borne diseases
Viral infections
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Zusammenfassung:Background In human leishmaniasis Th1/Th2 dichotomy similar to murine model is not clearly defined and surrogate marker(s) of protection is not yet known. In this study, Th1/Th2 cytokines (IL-5, IL-10, IL-13 and IFN-γ) profile induced by purified CD4+/CD8+ T cells in response to Leishmania antigens were assessed at transcript and protein levels in 14 volunteers with a history of self-healing cutaneous leishmaniasis (HCL) and compared with 18 healthy control volunteers. Methodology/Principal Findings CD4+/CD8+/CD14+ cells were purified from peripheral blood using magnetic beads; CD4+/CD8+ T cells were co-cultured with autologous CD14+ monocytes in the presence of soluble Leishmania antigens (SLA). Stimulation of either CD4+ T cells or CD8+ T cells of HCL volunteers with SLA induced a significantly (P
ISSN:1935-2727
1935-2735
DOI:10.1371/journal.pntd.0000845