Slit‐scan flow cytometer for recording simultaneous waveforms

Slit‐scan flow cytometer for recording simultaneous waveforms

We have developed a slit‐scan flow cytometer for recording the transient, simultaneous light scattering and fluorescence waveforms from individual metaphase chromosomes flowing through a focused laser beam. The two waveforms are recorded on a single‐memory waveform recorder by delaying one of the wa...

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Veröffentlicht in:Rev. Sci. Instrum.; (United States) 1985-05, Vol.56 (5), p.691-695
Hauptverfasser: Johnston, R. G., Bartholdi, M. F., Hiebert, R. D., Parson, J. D., Cram, L. S.
Format: Artikel
Sprache:eng
Schlagworte:
440300 - Miscellaneous Instruments- (-1989)
Biological and medical sciences
CHROMOSOMES
Cytogenetics
ELECTROMAGNETIC RADIATION
ELECTRONIC CIRCUITS
ELECTRONIC EQUIPMENT
FLUORESCENCE
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
LASER RADIATION
LIGHT SCATTERING
LUMINESCENCE
MEASURING METHODS
Methods and miscellaneous
OTHER INSTRUMENTATION
RADIATIONS
RECORDING SYSTEMS
SCATTERING
WAVE FORMS
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Zusammenfassung:We have developed a slit‐scan flow cytometer for recording the transient, simultaneous light scattering and fluorescence waveforms from individual metaphase chromosomes flowing through a focused laser beam. The two waveforms are recorded on a single‐memory waveform recorder by delaying one of the waveforms and switching between them. The light scattering and fluorescence waveforms contain complementary information on chromosome structure. The delay and switching electronics developed here may be useful in other contexts.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.1138207