Development of Surface Plasmon Resonance-Based Immunoassay for Aflatoxin B1

Aflatoxins are a group of highly toxic fungal secondary metabolites that occur in Aspergillus species and may contaminate foodstuffs and feeds. Two different anti-aflatoxin B1 antibodies were examined to develop a surface plasmon resonance (SPR)-based immunoassay to aflatoxin B1. A conjugate consist...

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Veröffentlicht in:Journal of agricultural and food chemistry 2000-11, Vol.48 (11), p.5097-5104
Hauptverfasser: Daly, Stephen J, Keating, Gary J, Dillon, Paul P, Manning, Bernadette M, O'Kennedy, Richard, Lee, Heather A, Morgan, Michael R. A
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Sprache:eng
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Zusammenfassung:Aflatoxins are a group of highly toxic fungal secondary metabolites that occur in Aspergillus species and may contaminate foodstuffs and feeds. Two different anti-aflatoxin B1 antibodies were examined to develop a surface plasmon resonance (SPR)-based immunoassay to aflatoxin B1. A conjugate consisting of aflatoxin B1−bovine serum albumin (BSA) was immobilized on the dextran gel surface. Competition between immobilized aflatoxin B1 conjugate and free aflatoxin B1 in solution for binding to antibody injected over the surface formed the basis for the assay. Regeneration of the antibody from the immobilized conjugate surface is essential for the development of such an inhibitive immunoassay. Problems were encountered with the regeneration of the sensor surface, due to the high-affinity binding of the antibodies. Conventional regeneration solutions consisting of low concentrations of NaOH and HCl worked to a degree, but regeneration was at the expense of the integrity of the immobilized conjugate. A polyclonal anti-aflatoxin B1 antibody was produced and was found to be regenerable using an organic solution consisting of 1 M ethanolamine with 20% (v/v) acetonitrile, pH 12.0. This combined high ionic strength and extreme pH, as well as chaotrophic properties and allowed the development of an inhibitive immunoassay. The assay had a linear range of 3.0−98.0 ng mL-1 with good reproducibility. Keywords: Aflatoxin B1; surface plasmon resonance; regeneration; inhibitive immunoassay
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9911693