Free space uptake and influx of Ni2+ in excised barley roots [Ion binding]
The free space uptake and influx of Ni2+ (63Ni2+) was characterized in excised roots of 7‐day‐old, dark‐grown barley (Hordeum vulgare L. cv. Møyar). Most of the Ni2+ (63Ni2+) present in the roots after a typical experiment was removed by washing in CaCl2+, unlabelled NiCl2 or EDTA solutions. The amo...
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Veröffentlicht in: | Physiologia plantarum 1986-12, Vol.68 (4), p.583-588 |
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Sprache: | eng |
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Zusammenfassung: | The free space uptake and influx of Ni2+ (63Ni2+) was characterized in excised roots of 7‐day‐old, dark‐grown barley (Hordeum vulgare L. cv. Møyar). Most of the Ni2+ (63Ni2+) present in the roots after a typical experiment was removed by washing in CaCl2+, unlabelled NiCl2 or EDTA solutions. The amount of loosely bound Ni2+ increased during the first few minutes in the uptake solution, and then remained constant. A small fraction was tightly bound, probably mainly in the cell walls. Binding, both loose and tight, increased with concentration, as did influx. However, the lower the external Ni2+ concentration, the larger was the proportion of Ni2+ tightly bound compared with the total amount of Ni2+ taken up. Ni2+ influx was lowered by 85% with 2,4‐dinitrophenol, which may indicate that Ni2+ is actively absorbed across the plasmalemma. |
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ISSN: | 0031-9317 1399-3054 |
DOI: | 10.1111/j.1399-3054.1986.tb03400.x |