Effect of inhibition of phenylpropanoid biosynthesis on peroxidase and IAA-oxidase activities and auxin content in alfalfa suspension cultures

The inhibition of phenylpropanoid biosynthesis by 2-aminoindan-2-phosphonic acid (AIP) in alfalfa suspension cultures (Medicago sativa L.) was associated with a marked decline in the content of cinnamic acid derivatives during the whole subculture interval. The levels of all investigated forms of ph...

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Veröffentlicht in:Plant physiology and biochemistry 2000-12, Vol.38 (12), p.949-956
Hauptverfasser: Hrubcova, M. (Academy of Science of the Czech Republic, Praha (Republique Tcheque). Institute of Experimental Botany), Cvikrova, M, Eder, J, Zon, J, Machackova, I
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Sprache:eng
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Zusammenfassung:The inhibition of phenylpropanoid biosynthesis by 2-aminoindan-2-phosphonic acid (AIP) in alfalfa suspension cultures (Medicago sativa L.) was associated with a marked decline in the content of cinnamic acid derivatives during the whole subculture interval. The levels of all investigated forms of phenolic acids in AIP-treated cells were longer than those of control with the exception of free phenolic acids on days 1 and 2. The dynamics of free IAA content in AIP-treated cells was similar to that of the control but its peak was significantly longer. The auxin content in AIP-treated cells on days 9 and 11 represented only 35 and 40 % of control values, respectively. AIP-treatment stimulated soluble peroxidase (EC 1.11.1.7) activity in the first 2 d after cell inoculation. Time-course of soluble IAA-oxidase activities was similar to that of soluble peroxidases (with the exception of the drop in peroxidase activity on day 3) but beginning day 6, the enzyme activities in AIP-treated cells were significantly longer until the end of the subculture interval.
ISSN:0981-9428
1873-2690
DOI:10.1016/S0981-9428(00)01208-0