Correlation between energy‐dependent tritium incorporation into CF1 and light‐induced protonation of thylakoid membranes

DCCD effects on proton titration of thylakoid membranes and light‐induced tritium incorporation in CF1 were compared. The pool of ionogenic groups exposed in the light (about 0.22 μmol · mg Chl−1 in the pH range 7–9) and tritiation of CF1 were both blocked by DCCD. The results are discussed with ref...

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Veröffentlicht in:	FEBS letters 1986-03, Vol.197 (1-2), p.125-128
Hauptverfasser:	Zolotareva, E.K., Opanasenko, V.K., Zakharov, S.D., Kuzmina, V.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell structures and functions CF0 and CF1, integral membrane portion and catalytic part of the chloroplast ATP-synthase, respectively Chl, chlorophyll Chloroplast, photosynthetic membrane and photosynthesis DCCD, N,N '-dicyclohexylcarbodiimide Fundamental and applied biological sciences. Psychology Molecular and cellular biology NEM, N-ethylmaleimide PMS, phenazine methosulphate Thylakoid membrane H+ channel ATP-synthase 1H-3 H exchange Buffer capacity (Pea chloroplast) β, proton buffer capacity
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Zusammenfassung:	DCCD effects on proton titration of thylakoid membranes and light‐induced tritium incorporation in CF1 were compared. The pool of ionogenic groups exposed in the light (about 0.22 μmol · mg Chl−1 in the pH range 7–9) and tritiation of CF1 were both blocked by DCCD. The results are discussed with reference to the role of the stage of proton (tritium) translocation through the H+ channel of ATP‐synthase in these processes.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(86)80311-8