DETECTION OF β-OXALYL DIAMINO PROPIONIC ACID IN CALLUS CULTURES OF LATHYRUS SATIVUS

Seeds of Lathyrus sativus contain a non-protein amino acid β-N-oxalyl-L-x-β diaminopropionic acid (BOAA) which has neurotoxic property. Callus cultures were initiated from germinating seeds of L. sativus on basal modified B5 medium supplemented with 2,4-dichlorophenoxyacetic acid and kinetin. BOAA w...

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Veröffentlicht in:	Current science (Bangalore) 1989-02, Vol.58 (3), p.112-114
Hauptverfasser:	NARAYAN, S. BADRI, BHATT, D. P., BHATT, P. N., MEHTA, A. R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Biological and medical sciences Biotechnology Callus Diamino amino acids Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Imbibition Indian culture Methods. Procedures. Technologies Miscellaneous Plant cells and fungal cells Plants Seedlings Seeds
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Zusammenfassung:	Seeds of Lathyrus sativus contain a non-protein amino acid β-N-oxalyl-L-x-β diaminopropionic acid (BOAA) which has neurotoxic property. Callus cultures were initiated from germinating seeds of L. sativus on basal modified B5 medium supplemented with 2,4-dichlorophenoxyacetic acid and kinetin. BOAA was detected by TLC in ethanolic extract of the callus cultures. The UV spectrum of BOAA extracted from cultured tissues could be superimposed on that of standard BOAA. Quantitative analysis revealed that the BOAA content of cultured tissue was less than that of the embryo axis of intact dry seeds.
ISSN:	0011-3891