Composition of Transcription Factor B-TFIID

Initiation of transcription by RNA polymerase II requires a TFIID factor, which can recognize the TATA element common to many promoters. Two distinct multisubunit TFIID factors can be resolved from extracts of mammalian cells, and both of them contain the well-characterized TATA-binding protein (TBP...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1992-09, Vol.89 (17), p.8140-8144
Hauptverfasser:	H. Th. Marc Timmers, Meyers, Rachel E., Sharp, Phillip A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - metabolism Antibodies Biochemistry Biological and medical sciences Cell extracts Chromatography DNA DNA-Binding Proteins - chemistry Fundamental and applied biological sciences. Psychology Gels HeLa Cells Humans Immunoprecipitation Molecular and cellular biology Molecular genetics Molecular Weight Precipitin Tests Ribonucleic acid RNA Silver staining TATA-Box Binding Protein Transcription Factor TFIID Transcription factors Transcription Factors - chemistry Transcription. Transcription factor. Splicing. Rna processing
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Zusammenfassung:	Initiation of transcription by RNA polymerase II requires a TFIID factor, which can recognize the TATA element common to many promoters. Two distinct multisubunit TFIID factors can be resolved from extracts of mammalian cells, and both of them contain the well-characterized TATA-binding protein (TBP) and are capable of supporting RNA polymerase II transcription in an in vitro reaction system. The smaller complex, B-TFIID, was purified and its subunit composition was determined. B-TFIID consists of two subunits: the TBP and a TBP-associated factor (TAF) of 170 kDa. This TAF is specific for B-TFIID and appears not to be present in the D-TFIID complex. Furthermore, it was found that the highly purified B-TFIID fractions have (d)ATPase activity.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.89.17.8140