A Normal Phase HPLC Method for the Determination of Two Arylmethylamino-Propanediols (Amaps), Crisnatol and 773U82 in Plasma

A normal phase HPLC method has been developed for the analysis of two potential antitumor agents, crisnatol and 773U82 in plasma samples. The assay procedures for both compounds are essentially the same. The assay uses a silica column and UV detection. The mobile phase consists of dichloromethane: methanol: perchloric acid (95:5:0.02). The plasma sample is extracted with chloroform: methanol (9:1) after potassium hydroxide basification. The reproducibility of the method was demonstrated by the analysis of spiked samples containing 5-1000 ng/ml. Application of the assay to analysis of crisnatol and 773U82 in plasma samples is demonstrated by measurement of the concentrations obtained from patients in Phase I clinical studies.