In vitro biosynthesis of a reserve glucomannan from Lilium testaceum

A particulate enzyme preparation, isolated from in vitro cultivated Lilium testaceum bulbs, was shown to catalyse the biosynthesis of a water-soluble β-1,4-glucomannan with a M r of 20 000. GDP[ 14C] mannose and GDP[ 14C] glucose were used as substrates for glucomannan biosynthesis. The incorporatio...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Phytochemistry (Oxford) 1991, Vol.30 (11), p.3579-3583
Hauptverfasser:	Wozniewski, Thomas, Blaschek, Wolfgang, Franz, Gerhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences bulbs Fundamental and applied biological sciences. Psychology glucomannan glucomannan-synthase in vitro cultivation Liliaceae Lilium testaceum Metabolism Photosynthesis, respiration. Anabolism, catabolism Plant physiology and development polysaccharide biosynthesis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A particulate enzyme preparation, isolated from in vitro cultivated Lilium testaceum bulbs, was shown to catalyse the biosynthesis of a water-soluble β-1,4-glucomannan with a M r of 20 000. GDP[ 14C] mannose and GDP[ 14C] glucose were used as substrates for glucomannan biosynthesis. The incorporation of[ 14C] mannose and/or [ 14C] glucose into the polymer was significantly stimulated by low M r glucomannan (3500). The isolated membrane preparation also exhibited GDP-mannose/-glucose-epimerase activity.
ISSN:	0031-9422 1873-3700
DOI:	10.1016/0031-9422(91)80069-D