Characterization of synthetic macroporous ion-exchange resins in low-pressure cartridges and columns : evaluation of the performance of Macro-Prep 50 S resin in the purification of anti-Klenow antibodies from goat serum
Three ion-exchange materials and one hydrophobic-interaction chromatography packing, based on a rigid macroporous polymer with large, relatively uniform pores, have been evaluated for low-pressure liquid chromatography of antibodies. These sorbents have high capacities for both small and large prote...
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Veröffentlicht in: | Journal of chromatography 1991-07, Vol.548 (1-2), p.165-178 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Three ion-exchange materials and one hydrophobic-interaction chromatography packing, based on a rigid macroporous polymer with large, relatively uniform pores, have been evaluated for low-pressure liquid chromatography of antibodies. These sorbents have high capacities for both small and large proteins and are mechanically, chemically, and thermally stable. Macro-Prep 50 S. CM and Q ion-exchange materials are strongly acidic, weakly acidic, and strongly basic, respectively. Protein binding and recovery, pressure-flow properties, and chemical and thermal stability were determined for each sorbent. A rapid, two-step method for the purification of anti-Klenow antibodies from goat serum was developed, based on the Macro-Prep 50 S strong-acid cation-exchange material and the Econo-Pac HIC prepacked hydrophobic-interaction cartridge. |
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ISSN: | 0021-9673 |
DOI: | 10.1016/S0021-9673(01)88599-X |