Mammalian expression of the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP) Characterization of the recombinant protein

A full-length 1,209 bp cDNA encoding the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP) was constructed and expressed in BHK-21 cells. The sequence agrees with the published gene and protein sequences. The cells were found to secrete SBP following transfection and G418 r...

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Veröffentlicht in:FEBS letters 1992-03, Vol.299 (1), p.23-27
Hauptverfasser: Hagen, Frederick S., Arguelles, Cesar, Sui, Li-ming, Zhang, Wei, Seidel, Paul R., Conroy, Stephen C., Petra, Philip H.
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Sprache:eng
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Zusammenfassung:A full-length 1,209 bp cDNA encoding the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP) was constructed and expressed in BHK-21 cells. The sequence agrees with the published gene and protein sequences. The cells were found to secrete SBP following transfection and G418 r selection. The recombinant protein binds 5α-dihydrotestosterone with a K d of 0.28 nM. It also binds testosterone and 17β-estradiol but not progesterone, estrone or cortisol revealing a steroid-binding specificity identical to that of human SBP, SDS-PAGE patterns are less complex than human SBP and show a monomeric molecular weight of about 43 kDa.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80091-T