Transcription-Driven Site-Specific DNA Recombination in vitro

Transcription of a topologically relaxed, circular DNA triggers recombination between two directly repeated res sites by γδ resolvase in vitro. This activation of recombination depends on the res site-to-site distance and the orientation of sites with respect to the direction of RNA polymerase track...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1993-04, Vol.90 (7), p.2759-2763
1. Verfasser: Droge, Peter
Format: Artikel
Sprache:eng
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Zusammenfassung:Transcription of a topologically relaxed, circular DNA triggers recombination between two directly repeated res sites by γδ resolvase in vitro. This activation of recombination depends on the res site-to-site distance and the orientation of sites with respect to the direction of RNA polymerase tracking. In addition to functioning as a site-specific recombinase, γδ resolvase acts as a site-specific topoisomerase and increases the topological linking number of templates during transcription. The data suggest that the link between transcription and recombination could be negative DNA supercoiling that transiently builds up on a relatively short DNA segment in the wake of an advancing RNA polymerase. Surprisingly, transcription-driven recombination is not inhibited by the presence of large amounts of eukaryotic topoisomerase type I, indicating that site-specific recombination can override relaxation by diffusible topoisomerases. This in vitro system might therefore serve as a model for some transcription-directed recombination events observed in vivo.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.7.2759