A New Method of Synthesis of Fluorescently Labelled Oligonucleotides and Their Application in DNA Sequencing

A new approach to the chemical synthesis of oligonucleotides bearing reporter functional groups based on the modification of cytosine residues during the final deprotection step is reported. The application of the fluorescently labelled primer for the automated DNA sequencing is shown.

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Bibliographische Detailangaben
Veröffentlicht in:	Nucleosides & nucleotides 1992-12, Vol.11 (10), p.1703-1711
Hauptverfasser:	Markiewicz, Wojciech T., Gröger, Gabriele, Rösch, Rudi, Zebrowska, Anna, Seliger, Hartmut
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbohydrates. Nucleosides and nucleotides Chemistry Exact sciences and technology Nucleosides, nucleotides and oligonucleotides Organic chemistry Preparations and properties
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A new approach to the chemical synthesis of oligonucleotides bearing reporter functional groups based on the modification of cytosine residues during the final deprotection step is reported. The application of the fluorescently labelled primer for the automated DNA sequencing is shown.
ISSN:	0732-8311 2332-3892
DOI:	10.1080/07328319208017817