Identification of bovine and porcine rotavirus G types by PCR

A new seminested PCR typing assay has been extended to identify the important veterinary rotavirus serotypes G5, G6, G10, and G11, as well as the rare human serotype G8. The specificity of the method was evaluated with 30 standard laboratory strains of the G1 to G6 and G8 to G11 types. Rotavirus str...

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Veröffentlicht in:	Journal of Clinical Microbiology 1994-05, Vol.32 (5), p.1338-1340
Hauptverfasser:	Gouvea, V, Santos, N, Timenetsky, M. do Carmo
Format:	Artikel
Sprache:	eng
Schlagworte:	AMPLIFICATION CHAINE POLYMERASE Animals Antigens, Viral Base Sequence Biological and medical sciences Capsid - genetics Capsid Proteins Cattle Cattle Diseases - microbiology DNA Primers - genetics DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Gastroenteritis - microbiology Gastroenteritis - veterinary Humans IDENTIFICACION IDENTIFICATION Microbiology Molecular Sequence Data Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data REACCION DE CADENAS DE POLIMERASA ROTAVIRUS Rotavirus - classification Rotavirus - genetics Rotavirus - isolation & purification Rotavirus Infections - microbiology Rotavirus Infections - veterinary Sensitivity and Specificity SEROTIPOS SEROTYPE Swine Swine Diseases - microbiology Techniques used in virology Virology
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Beschreibung
Zusammenfassung:	A new seminested PCR typing assay has been extended to identify the important veterinary rotavirus serotypes G5, G6, G10, and G11, as well as the rare human serotype G8. The specificity of the method was evaluated with 30 standard laboratory strains of the G1 to G6 and G8 to G11 types. Rotavirus strain types G6 and G8, not previously recognized in pigs, were identified in field specimens of porcine origin
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.32.5.1338-1340.1994