The role of α2‐adrenoceptors in the vasculature of the rat tail

1 The effects of α2‐adrenoceptor agonists and antagonists on rat tail skin temperature (tts), an indicator of local cutaneous blood flow, were studied in conscious and anaesthetized rats and in the isolated, Krebs perfused, vascular bed of the rat tail. 2 In conscious rats, at an ambient temperature of 18.5–20°C, tts was 21.0 ± 0.2°C and core (rectal) temperature (tc) was 38.2 ± 0.04°C (n = 126). The α2‐adrenoceptor antagonist, delequamine (RS‐15385‐197; 1 mg kg−1, s.c., n = 6), produced a rapid elevation in t to 29.1 ± 0.7°C (P < 0.001 vs. saline‐treated control group), attained 10 min after injection. tc fell slightly, by 1.0 ± 0.1°C. The tts response was dose‐related over the dose‐range tested (0.01‐1 mg kg‐1, s.c.), with an ED50 of 17 μg kg1, s.c. (n =6 per dose). 3 The maximum increases in tts in response to a dose of 1 mg kg‐1, s.c. of α2‐adrenoceptor antagonists were as follows (n = 6 per drug): delequamine (+ 9.6 ± 0.8°C)> yohimbine (+ 9.0 ± 1.0°C)> WY‐26703 (+ 7.9 ± 1.3°C) > piperoxan (+ 5.6 ± 1 7°C) > idazoxan (+ 4.6 ± 1.3°C) > imiloxan (+ 4.1 ± 1.3°C) > SKF 104078 (+2.0 ± 1.9°C)>BDF‐6143 (+1.3 ± 0.8°C). 4 Prazosin (0.3mg kg‐1, s.c.), hydralazine (10mg kg‐1, s.c.) and nifedipine (3mg kg‐1, s.c.) did not increase tts, whereas propranolol (10mg kg‐1, s.c.) evoked a small increase in tts (+2.9 ± 1.0°C). Pentolinium (2–10 mg kg−1, s.c.) elicited a dose‐related increase in tts which was elevated by 4.4 ± 1.3°C after a dose of 10 mg kg−1; tc was reduced in a dose‐related manner. Drug vehicles (1 ml kg−1, s.c) had no effect on tte or tc. 5 In anaesthetized rats, idazoxan (300 μg, i.v.) produced a rapid increase in tts which was detectable 2 min after beginning the injection, reaching a peak after 7 min. When the same dose was administered i.c.v., tts also rose, but more slowly. The peak response (+ 3.6 ± 0.7°C, n = 5) was significantly smaller than when idazoxan was administered intravenously (+6.3± 1.2°C, n = 5), which suggests that the increase in tts following systemic administration of α2‐adrenoceptor antagonists is not due to a central effect. The change in tts was not secondary to changes in blood pressure. 6 In the isolated, Krebs perfused, tail vascular bed of the rat, at an ambient temperature of 20–21°C, under constant flow conditions (3.5‐4.0 ml min−1; n = 4), baseline perfusion pressure was 57 ± 4 mmHg. 5‐Hydroxytryptamine (5‐HT; 70–150 nm) increased perfusion pressure by 56 ± 9 mmHg. The α2‐adrenoceptor agonist, UK‐14,304 (10 nmol), elicite