In Saccharomyces cerevisiae overexpression of hybrid virus-like-particles correlates with altered cell volume distributions

Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentat...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biotechnology letters 1994-11, Vol.16 (11), p.1131-1134
Hauptverfasser:	Cavadini, P, Popolo, L, Vai, M, Varona, R, Garofano, L, Vanoni, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology cytolysis fermentation Fundamental and applied biological sciences. Psychology gala gene gene expression genes Genetic engineering Genetic technics genetic transformation growth kinetics Methods. Procedures. Technologies Modification of gene expression level recombinant yeasts Saccharomyces cerevisiae virus-like particles
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose leads to galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF01020838