Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents

A fecal PCR assay for detection of Helicobacter infections in laboratory, rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not requ...

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Veröffentlicht in:	Journal of Clinical Microbiology 1997-06, Vol.35 (6), p.1620-1623
Hauptverfasser:	Beckwith, C.S, Franklin, C.L, Hook, R.R. Jr, Besch-Williford, C.L, Riley, L.K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal bacterial diseases animal diseases animal health Animals Bacterial diseases bacterial infections Biological and medical sciences Cecum - microbiology DNA, Bacterial - analysis Feces - microbiology Helicobacter Helicobacter - genetics Helicobacter - isolation & purification Helicobacter Infections - diagnosis Helicobacter Infections - microbiology Infectious diseases laboratory animals Medical sciences Mice Mice, Inbred A Mice, Inbred BALB C Polymerase Chain Reaction - methods RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	A fecal PCR assay for detection of Helicobacter infections in laboratory, rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of rodents, which is especially important for valuable rodents, such as transgenic mice.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.35.6.1620-1623.1997