Current Response and Determination of Traces of Coenzyme I At a Silver Microelectrode

A useful current response of nicotinamide coenzyme I at a silver microelectrode was obtained and, therefore, an electrochemical method has been developed for the determination of traces of the coenzyme by square wave adsorption stripping voltammetry. Under optimum conditions the proposed method provides a linear response over the coenzyme concentration in the range of 0.5∼7.5 μmol/L. The detection limit was 0.2 μmol/L. The relative standard deviation was 3.5% for 6 successive determinations of the coenzyme at 1.0 μmol/L. In view of the merits of the electrochemical techniques and this microelectrode, this method will be of great usefulness in the biochemical and clinical fields in the future.