Improvement of amperometric glucose biosensor by the immobilization of FcCD inclusive complex and carbon nanotube

A novel glucose biosensor was constructed by using ferrocene-carbonyl-β-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. FcCD inclusive complex and glucose oxidase (GOx) were covalently bonded to CNTs by poly- l -lysine (P...

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Veröffentlicht in:Analyst (London) 2010-06, Vol.135 (6), p.1339-1344
Hauptverfasser: Zheng, Longzhen, Li, Junhua, Xu, Jingpeng, Xiong, Leyan, Zheng, Dan, Liu, Qiang, Liu, Wen, Li, Yindi, Yang, Shaoming, Xia, Jian
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Sprache:eng
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Zusammenfassung:A novel glucose biosensor was constructed by using ferrocene-carbonyl-β-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. FcCD inclusive complex and glucose oxidase (GOx) were covalently bonded to CNTs by poly- l -lysine (PLL) to fabricate a glucose biosensor. The electrocatalytic oxidation of glucose at the biosensor occurred at low potential below 200 mV, avoiding the interference of the main interfering substances in real samples containing a 5-times higher concentration of l -cysteine, ascorbic acid, and uric acid. The biosensor showed fast response for glucose. A broad linear range of glucose concentration from 1.0 × 10 −5 to 2.90 × 10 −3 M was obtained and the detection limit was 2.2 × 10 −6 M. A novel glucose biosensor was constructed by using ferrocene-carbonyl-β-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter.
ISSN:0003-2654
1364-5528
DOI:10.1039/b925505b