Improvement of amperometric glucose biosensor by the immobilization of FcCD inclusive complex and carbon nanotube
A novel glucose biosensor was constructed by using ferrocene-carbonyl-β-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. FcCD inclusive complex and glucose oxidase (GOx) were covalently bonded to CNTs by poly- l -lysine (P...
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Veröffentlicht in: | Analyst (London) 2010-06, Vol.135 (6), p.1339-1344 |
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Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A novel glucose biosensor was constructed by using ferrocene-carbonyl-β-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. FcCD inclusive complex and glucose oxidase (GOx) were covalently bonded to CNTs by poly-
l
-lysine (PLL) to fabricate a glucose biosensor. The electrocatalytic oxidation of glucose at the biosensor occurred at low potential below 200 mV, avoiding the interference of the main interfering substances in real samples containing a 5-times higher concentration of
l
-cysteine, ascorbic acid, and uric acid. The biosensor showed fast response for glucose. A broad linear range of glucose concentration from 1.0 × 10
−5
to 2.90 × 10
−3
M was obtained and the detection limit was 2.2 × 10
−6
M.
A novel glucose biosensor was constructed by using ferrocene-carbonyl-β-cyclodextrin (FcCD) inclusive complex as electron-transfer mediator and carbon nanotubes (CNTs) as electron-transfer promoter. |
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ISSN: | 0003-2654 1364-5528 |
DOI: | 10.1039/b925505b |