Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use as drug resistance marker for transformation

The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 tr...

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Veröffentlicht in:	Applied and Environmental Microbiology 1998-05, Vol.64 (5), p.1947-1949
Hauptverfasser:	Kim, I.G. (Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon.), Nam, S.K, Sohn, J.H, Rhee, S.K, An, G.H, Lee, S.H, Choi, E.S
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Bacteria Biological and medical sciences Biotechnology Cloning Cloning, Molecular Cycloheximide - pharmacology DNA Drug resistance Drug Resistance, Microbial ELECTROPORACION ELECTROPORATION Fundamental and applied biological sciences. Psychology GENBANK/AF004672 GENBANK/AF016256 Genes Genes, Fungal Genetic engineering GENETIC MARKERS Genetic technics GENETIC TRANSFORMATION Genetics and Molecular Biology LEVADURA LEVURE MARCADORES GENETICOS MARKER GENES MARQUEUR GENETIQUE Methods. Procedures. Technologies Microbiology Miscellaneous MOLECULAR SEQUENCE DATA NUCLEOTIDE SEQUENCE Phaffia rhodozyma Plasmids Polymerase Chain Reaction Proteins Ribosomal Proteins - genetics RIBOSOMAS RIBOSOME RIBOSOMES SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE Synthetic digonucleotides and genes. Sequencing TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic Yeast YEASTS Yeasts - drug effects Yeasts - genetics
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Beschreibung
Zusammenfassung:	The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/micrograms of DNA with an integrated copy number of about seven per haploid genome
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.64.5.1947-1949.1998