Development of gateway binary vectors, R4L1pGWBs, for promoter analysis in higher plants

We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gatewa...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-11, Vol.73 (11), p.2556-2559
Hauptverfasser:	Nakamura, S., Shimane Univ., Matsue (Japan). Center for Integrated Research in Science, Nakao, A, Kawamukai, M, Kimura, T, Ishiguro, S, Nakagawa, T
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN RECOMBINADO ADN RECOMBINE Amino Acid Sequence Base Sequence binary vector Biological and medical sciences BIOTECHNOLOGIE VEGETALE BIOTECNOLOGIA VEGETAL CLONACION CLONAGE CLONING Cloning, Molecular DNA, Complementary - genetics FLUORESCENCE FLUORESCENCIA Fundamental and applied biological sciences. Psychology Gateway cloning Genes, Reporter - genetics Genetic Engineering - methods GENETIC TRANSFORMATION Genetic Vectors - genetics http://www.fao.org/aos/agrovoc#c_10935 http://www.fao.org/aos/agrovoc#c_15952 http://www.fao.org/aos/agrovoc#c_16014 http://www.fao.org/aos/agrovoc#c_27590 http://www.fao.org/aos/agrovoc#c_27594 http://www.fao.org/aos/agrovoc#c_27619 http://www.fao.org/aos/agrovoc#c_3220 http://www.fao.org/aos/agrovoc#c_8164 PLANT BIOTECHNOLOGY plant transformation PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants - genetics PLASMIDE PLASMIDIOS PLASMIDS promoter Promoter Regions, Genetic - genetics RECOMBINANT DNA reporter Time Factors TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic TRANSGENIC PLANTS VECTEUR DE MALADIE VECTORES VECTORS
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Zusammenfassung:	We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoter-attB1-reporter construct. The reporters employed in R4L1pGWBs were β-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.90720