Differential Coupling of μ‐, δ‐, and κ‐Opioid Receptors to Gα16‐Mediated Stimulation of Phospholipase C

: The μ‐opioid receptor has recently been shown to stimulate phosphoinositide‐specific phospholipase C via the pertussis toxin‐sensitive G16 protein. Given the promiscuous nature of G16 and the high degree of resemblance of signaling properties of the three opioid receptors, both δ‐ and κ‐opioid receptors are likely to activate G16. Interactions of δ‐ and κ‐opioid receptors with G16 were examined by coexpressing the opioid receptors and Gα16 in COS‐7 cells. The δ‐selective agonist [d‐Pen2,d‐Pen5]enkephalin potently stimulated the formation of inositol phosphates in cells coexpressing the δ‐opioid receptor and Gα16. The δ‐opioid receptor‐mediated stimulation of phospholipase C was absolutely dependent on the coexpression of simeter for quality control of blood units and irradiators. 13. Transfusion 1993;33: 898–901.14. Butson MJ, Yu PK, Cheung T, et al. Dosimetry of blood irradiation with radiochromic film. Transfus Med 1999;9: 205–8.15. Nath R, Biggs PJ, Ling CC, et al. AAPM code of practice for radiotherapy accelerators: Report of AAPM Radiation Therapy Task Group No. 45. Med Phys