Cultivation of Methanogens under Low-Hydrogen Conditions by Using the Coculture Method

We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture e...

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Veröffentlicht in:	Applied and Environmental Microbiology 2009-07, Vol.75 (14), p.4892-4896
Hauptverfasser:	Sakai, Sanae, Imachi, Hiroyuki, Sekiguchi, Yuji, Tseng, I. Cheng, Ohashi, Akiyoshi, Harada, Hideki, Kamagata, Yoichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteria - classification Bacteria - genetics Bacteria - growth & development Bacteria - metabolism Biological and medical sciences Cluster Analysis Coculture Techniques - methods DNA, Archaeal - chemistry DNA, Archaeal - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Environmental Microbiology Fundamental and applied biological sciences. Psychology Hydrogen Hydrogen - metabolism Methane - metabolism Methanomicrobiales Microbial Ecology Microbiology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA
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Zusammenfassung:	We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and eventually isolated a new methanogen, within the order METHANOMICROBIALES:
ISSN:	0099-2240 1098-5336 1098-6596
DOI:	10.1128/AEM.02835-08