Conditions for natural transformation of Ralstonia solanacearum

The development of competence allowing natural transformation of Ralstonia solanacearum was found to occur during exponential growth and not in response to any excreted factors. Linear DNAs were effectively integrated by recombination requiring a minimum of 50 bp of homologous DNA. Therefore, DNA fr...

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Veröffentlicht in:	Applied and Environmental Microbiology 1997-12, Vol.63 (12), p.4965-4968
Hauptverfasser:	Bertolla, F, Gijsegem, F. van, Nesme, X, Simonet, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriology Biological and medical sciences Deoxyribonucleic acid DNA DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Bacterial - metabolism Ecology Fundamental and applied biological sciences. Psychology GENETIC TRANSFORMATION Genetics Gram-Negative Aerobic Rods and Cocci - genetics Gram-Negative Aerobic Rods and Cocci - growth & development Gram-Negative Aerobic Rods and Cocci - metabolism Life Sciences Microbiology Models, Genetic Molecular Weight PSEUDOMONAS SOLANACEARUM Sequence Homology, Nucleic Acid TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic
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Beschreibung
Zusammenfassung:	The development of competence allowing natural transformation of Ralstonia solanacearum was found to occur during exponential growth and not in response to any excreted factors. Linear DNAs were effectively integrated by recombination requiring a minimum of 50 bp of homologous DNA. Therefore, DNA from other genera and species were ineffective
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.63.12.4965-4968.1997