A Direct Injection Assay of Angiotensin Converting Enzyme in Tissue Extracts

A rapid, sensitive and selective method for the determination of angiotensin converting enzyme (ACE) activity in tissue extract and serum is described. The procedure is based on the high performance liquid chromatographic separation of the synthetic substrate furylacryloylphenylalanyl-glycyl-glycine...

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Veröffentlicht in:	Journal of liquid chromatography & related technologies 1997-12, Vol.20 (20), p.3309-3315
Hauptverfasser:	Smiley, J. W., Doig, M. T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A rapid, sensitive and selective method for the determination of angiotensin converting enzyme (ACE) activity in tissue extract and serum is described. The procedure is based on the high performance liquid chromatographic separation of the synthetic substrate furylacryloylphenylalanyl-glycyl-glycine (FAPGG) from the hydrolysis product furylacryloylphenylalanine (FAP). Baseline separation is accomplished in ten minutes by direct injection of biological assay mixtures onto a 15 cm shielded hydrophobic phase column with isocratic elution using 180 mM ammonium acetate/ 15% acetonitrile as the mobile phase. Both substrate and product are detected by absorbance at 305 nm.
ISSN:	1082-6076 1520-572X
DOI:	10.1080/10826079708005832