Identification of Coagulase-Negative Staphylococci by Using the BD Phoenix System in the Low-Inoculum Mode

The new "low-inoculum" mode of the Phoenix system was evaluated to identify clinical coagulase-negative staphylococci. API ID32 Staph panels were used as comparators, and discrepancies were resolved by 16S rRNA and tuf gene analysis. The system correctly identified 90.5% of isolates, with...

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Veröffentlicht in:	Journal of Clinical Microbiology 2008-11, Vol.46 (11), p.3826-3828
Hauptverfasser:	Brigante, Gioconda, Menozzi, Maria Grazia, Pini, Beatrice, Porta, Rosaria, Somenzi, Pietro, Sciacca, Agata, Spanu, Teresa, Stefani, Stefania
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Typing Techniques - methods Bacteriology Biological and medical sciences Coagulase - biosynthesis Fundamental and applied biological sciences. Psychology Humans Microbiology Peptide Elongation Factor Tu - genetics RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Staphylococcal Infections - diagnosis Staphylococcal Infections - microbiology Staphylococcus - classification Staphylococcus - enzymology Staphylococcus - isolation & purification
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Zusammenfassung:	The new "low-inoculum" mode of the Phoenix system was evaluated to identify clinical coagulase-negative staphylococci. API ID32 Staph panels were used as comparators, and discrepancies were resolved by 16S rRNA and tuf gene analysis. The system correctly identified 90.5% of isolates, with a mean time of 10.2 h. Accuracy was satisfactory for Staphylococcus epidermidis, S. saprophyticus, and S. haemolyticus.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.00877-08