Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains

Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 S...

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Veröffentlicht in:Applied and Environmental Microbiology 2008-04, Vol.74 (7), p.2153-2160
Hauptverfasser: Coombes, Brian K, Wickham, Mark E, Mascarenhas, Mariola, Gruenheid, Samantha, Finlay, B. Brett, Karmali, Mohamed A
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container_end_page 2160
container_issue 7
container_start_page 2153
container_title Applied and Environmental Microbiology
container_volume 74
creator Coombes, Brian K
Wickham, Mark E
Mascarenhas, Mariola
Gruenheid, Samantha
Finlay, B. Brett
Karmali, Mohamed A
description Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 STEC serotypes that are globally distributed, several of which are associated with outbreaks and/or severe human illness such as hemolytic-uremic syndrome (HUS) and hemorrhagic colitis. At present, the underlying genetic basis of virulence potential in non-O157 STEC is unknown, although horizontal gene transfer and the acquisition of new pathogenicity islands are an expected origin. We used seropathotype classification as a framework to identify genetic elements that distinguish non-O157 STEC strains posing a serious risk to humans from STEC strains that are not associated with severe and epidemic disease. We report the identification of three genomic islands encoding non-LEE effector (nle) genes and 14 individual nle genes in non-O157 STEC strains that correlate independently with outbreak and HUS potential in humans. The implications for transmissible zoonotic spread and public health are discussed. These results and methods offer a molecular risk assessment strategy to rapidly recognize and respond to non-O157 STEC strains from environmental and animal sources that might pose serious public health risks to humans.
doi_str_mv 10.1128/AEM.02566-07
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source American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection
subjects Animals
Biological and medical sciences
Cattle
Colon - microbiology
Disease transmission
E coli
Epidemics
Escherichia coli
Escherichia coli Infections - diagnosis
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genomic Islands - genetics
Genomic Islands - physiology
Hemolytic-Uremic Syndrome - epidemiology
Hemolytic-Uremic Syndrome - microbiology
Humans
Microbiology
Molecular biology
Public Health
Public Health Microbiology
Shiga Toxins - biosynthesis
Shiga-Toxigenic Escherichia coli - classification
Shiga-Toxigenic Escherichia coli - genetics
Shiga-Toxigenic Escherichia coli - isolation & purification
Virulence Factors - genetics
title Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains
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