Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains
Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 S...
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Veröffentlicht in: | Applied and Environmental Microbiology 2008-04, Vol.74 (7), p.2153-2160 |
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creator | Coombes, Brian K Wickham, Mark E Mascarenhas, Mariola Gruenheid, Samantha Finlay, B. Brett Karmali, Mohamed A |
description | Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 STEC serotypes that are globally distributed, several of which are associated with outbreaks and/or severe human illness such as hemolytic-uremic syndrome (HUS) and hemorrhagic colitis. At present, the underlying genetic basis of virulence potential in non-O157 STEC is unknown, although horizontal gene transfer and the acquisition of new pathogenicity islands are an expected origin. We used seropathotype classification as a framework to identify genetic elements that distinguish non-O157 STEC strains posing a serious risk to humans from STEC strains that are not associated with severe and epidemic disease. We report the identification of three genomic islands encoding non-LEE effector (nle) genes and 14 individual nle genes in non-O157 STEC strains that correlate independently with outbreak and HUS potential in humans. The implications for transmissible zoonotic spread and public health are discussed. These results and methods offer a molecular risk assessment strategy to rapidly recognize and respond to non-O157 STEC strains from environmental and animal sources that might pose serious public health risks to humans. |
doi_str_mv | 10.1128/AEM.02566-07 |
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Brett ; Karmali, Mohamed A</creator><creatorcontrib>Coombes, Brian K ; Wickham, Mark E ; Mascarenhas, Mariola ; Gruenheid, Samantha ; Finlay, B. Brett ; Karmali, Mohamed A</creatorcontrib><description>Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 STEC serotypes that are globally distributed, several of which are associated with outbreaks and/or severe human illness such as hemolytic-uremic syndrome (HUS) and hemorrhagic colitis. At present, the underlying genetic basis of virulence potential in non-O157 STEC is unknown, although horizontal gene transfer and the acquisition of new pathogenicity islands are an expected origin. We used seropathotype classification as a framework to identify genetic elements that distinguish non-O157 STEC strains posing a serious risk to humans from STEC strains that are not associated with severe and epidemic disease. We report the identification of three genomic islands encoding non-LEE effector (nle) genes and 14 individual nle genes in non-O157 STEC strains that correlate independently with outbreak and HUS potential in humans. The implications for transmissible zoonotic spread and public health are discussed. These results and methods offer a molecular risk assessment strategy to rapidly recognize and respond to non-O157 STEC strains from environmental and animal sources that might pose serious public health risks to humans.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.02566-07</identifier><identifier>PMID: 18245257</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Colon - microbiology ; Disease transmission ; E coli ; Epidemics ; Escherichia coli ; Escherichia coli Infections - diagnosis ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genomic Islands - genetics ; Genomic Islands - physiology ; Hemolytic-Uremic Syndrome - epidemiology ; Hemolytic-Uremic Syndrome - microbiology ; Humans ; Microbiology ; Molecular biology ; Public Health ; Public Health Microbiology ; Shiga Toxins - biosynthesis ; Shiga-Toxigenic Escherichia coli - classification ; Shiga-Toxigenic Escherichia coli - genetics ; Shiga-Toxigenic Escherichia coli - isolation & purification ; Virulence Factors - genetics</subject><ispartof>Applied and Environmental Microbiology, 2008-04, Vol.74 (7), p.2153-2160</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Apr 2008</rights><rights>Copyright © 2008, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-bc303b63109f169cfbba45cd000991711c18d3afe24587c9bdbc5ceb440f9cca3</citedby><cites>FETCH-LOGICAL-c563t-bc303b63109f169cfbba45cd000991711c18d3afe24587c9bdbc5ceb440f9cca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292595/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292595/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20245975$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18245257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coombes, Brian K</creatorcontrib><creatorcontrib>Wickham, Mark E</creatorcontrib><creatorcontrib>Mascarenhas, Mariola</creatorcontrib><creatorcontrib>Gruenheid, Samantha</creatorcontrib><creatorcontrib>Finlay, B. Brett</creatorcontrib><creatorcontrib>Karmali, Mohamed A</creatorcontrib><title>Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 STEC serotypes that are globally distributed, several of which are associated with outbreaks and/or severe human illness such as hemolytic-uremic syndrome (HUS) and hemorrhagic colitis. At present, the underlying genetic basis of virulence potential in non-O157 STEC is unknown, although horizontal gene transfer and the acquisition of new pathogenicity islands are an expected origin. We used seropathotype classification as a framework to identify genetic elements that distinguish non-O157 STEC strains posing a serious risk to humans from STEC strains that are not associated with severe and epidemic disease. We report the identification of three genomic islands encoding non-LEE effector (nle) genes and 14 individual nle genes in non-O157 STEC strains that correlate independently with outbreak and HUS potential in humans. The implications for transmissible zoonotic spread and public health are discussed. These results and methods offer a molecular risk assessment strategy to rapidly recognize and respond to non-O157 STEC strains from environmental and animal sources that might pose serious public health risks to humans.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Colon - microbiology</subject><subject>Disease transmission</subject><subject>E coli</subject><subject>Epidemics</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - diagnosis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genomic Islands - genetics</subject><subject>Genomic Islands - physiology</subject><subject>Hemolytic-Uremic Syndrome - epidemiology</subject><subject>Hemolytic-Uremic Syndrome - microbiology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Molecular biology</subject><subject>Public Health</subject><subject>Public Health Microbiology</subject><subject>Shiga Toxins - biosynthesis</subject><subject>Shiga-Toxigenic Escherichia coli - classification</subject><subject>Shiga-Toxigenic Escherichia coli - genetics</subject><subject>Shiga-Toxigenic Escherichia coli - isolation & purification</subject><subject>Virulence Factors - genetics</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1v1DAQxSMEokvhxhkMEpxIGdtxnFyQVtVCkVpase3ZcibOxsUbFzuB9r_HYVflQ7Lkg39-8968LHtO4YhSVr1frs6OgImyzEE-yBYU6ioXnJcPswVAXeeMFXCQPYnxGgAKKKvH2QGtWCGYkIvs9sw7g5PTgSwH7e6ijUSnM5ClbcmlJ8sYTYxk7A25mBpnkZwY7caefLXxG_Ed-eKH_JwKSda93ej05dYO-UXw7YR22JBVxN4Ei73VBL2zZD0GbYf4NHvUaRfNs_19mF19XF0en-Sn558-Hy9PcxQlH_MGOfCm5ClWR8sau6bRhcAW5mxUUoq0arnuTApUSaybtkGBpikK6GpEzQ-zDzvdm6nZmhbNkOY7dRPsVoc75bVV_74Mtlcb_0MxVjNRiyTwdi8Q_PfJxFFtbUTjnB6Mn6JiUJWUMZrA1_-B134KaakzI2pZlsAT9G4HYfAxBtPdO6Gg5j5V6lP97lOBTPiLv93_gfcFJuDNHtARteuCHtDGe45B4mo5p3i141JJ_U8bjNJxq7TZKlkoqRgVs7eXO6bTXulNSDpXawaUA1SySlL8F4rXvEY</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Coombes, Brian K</creator><creator>Wickham, Mark E</creator><creator>Mascarenhas, Mariola</creator><creator>Gruenheid, Samantha</creator><creator>Finlay, B. Brett</creator><creator>Karmali, Mohamed A</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7U7</scope><scope>5PM</scope></search><sort><creationdate>20080401</creationdate><title>Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains</title><author>Coombes, Brian K ; Wickham, Mark E ; Mascarenhas, Mariola ; Gruenheid, Samantha ; Finlay, B. Brett ; Karmali, Mohamed A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c563t-bc303b63109f169cfbba45cd000991711c18d3afe24587c9bdbc5ceb440f9cca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Colon - microbiology</topic><topic>Disease transmission</topic><topic>E coli</topic><topic>Epidemics</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - diagnosis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genomic Islands - genetics</topic><topic>Genomic Islands - physiology</topic><topic>Hemolytic-Uremic Syndrome - epidemiology</topic><topic>Hemolytic-Uremic Syndrome - microbiology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Molecular biology</topic><topic>Public Health</topic><topic>Public Health Microbiology</topic><topic>Shiga Toxins - biosynthesis</topic><topic>Shiga-Toxigenic Escherichia coli - classification</topic><topic>Shiga-Toxigenic Escherichia coli - genetics</topic><topic>Shiga-Toxigenic Escherichia coli - isolation & purification</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coombes, Brian K</creatorcontrib><creatorcontrib>Wickham, Mark E</creatorcontrib><creatorcontrib>Mascarenhas, Mariola</creatorcontrib><creatorcontrib>Gruenheid, Samantha</creatorcontrib><creatorcontrib>Finlay, B. 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Brett</au><au>Karmali, Mohamed A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>74</volume><issue>7</issue><spage>2153</spage><epage>2160</epage><pages>2153-2160</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 STEC serotypes that are globally distributed, several of which are associated with outbreaks and/or severe human illness such as hemolytic-uremic syndrome (HUS) and hemorrhagic colitis. At present, the underlying genetic basis of virulence potential in non-O157 STEC is unknown, although horizontal gene transfer and the acquisition of new pathogenicity islands are an expected origin. We used seropathotype classification as a framework to identify genetic elements that distinguish non-O157 STEC strains posing a serious risk to humans from STEC strains that are not associated with severe and epidemic disease. We report the identification of three genomic islands encoding non-LEE effector (nle) genes and 14 individual nle genes in non-O157 STEC strains that correlate independently with outbreak and HUS potential in humans. The implications for transmissible zoonotic spread and public health are discussed. These results and methods offer a molecular risk assessment strategy to rapidly recognize and respond to non-O157 STEC strains from environmental and animal sources that might pose serious public health risks to humans.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>18245257</pmid><doi>10.1128/AEM.02566-07</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biological and medical sciences Cattle Colon - microbiology Disease transmission E coli Epidemics Escherichia coli Escherichia coli Infections - diagnosis Fundamental and applied biological sciences. Psychology Genes, Bacterial Genomic Islands - genetics Genomic Islands - physiology Hemolytic-Uremic Syndrome - epidemiology Hemolytic-Uremic Syndrome - microbiology Humans Microbiology Molecular biology Public Health Public Health Microbiology Shiga Toxins - biosynthesis Shiga-Toxigenic Escherichia coli - classification Shiga-Toxigenic Escherichia coli - genetics Shiga-Toxigenic Escherichia coli - isolation & purification Virulence Factors - genetics |
title | Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains |
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