Pooling Evidence to Identify Cell Cycle–Regulated Genes

Most of the biological studies have embraced statistical approaches to make inferences. It is common to have several independent experiments to test the same null hypothesis. The goal of research on pooling evidence is to combine the results of these tests to ask if there is evidence from the collec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Zheng, Gaolin, Milledge, Tom, George, E. Olusegun, Narasimhan, Giri
Format: Buchkapitel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Most of the biological studies have embraced statistical approaches to make inferences. It is common to have several independent experiments to test the same null hypothesis. The goal of research on pooling evidence is to combine the results of these tests to ask if there is evidence from the collection of studies to reject the null hypothesis. In this study, we evaluated four different pooling techniques (Fisher, Logit, Stouffer and Liptak) to combine the evidence from independent microarray experiments in order to identify cell cycle-regulated genes. We were able to identify a better set of cell cycle-regulated genes using the pooling techniques based on our benchmark study on budding yeast (Saccharomyces cerevisiae). Our gene ontology study on time series data of both the budding yeast and the fission yeast (Schizosaccharomyces pombe) showed that the GO terms that are related to cell cycle are significantly enriched in the cell cycle-regulated genes identified using pooling techniques.
ISSN:0302-9743
1611-3349
DOI:10.1007/11758525_94